枸杞果多糖对哮喘小鼠炎症损伤影响及作用机制
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(1.宁夏医科大学基础医学院,宁夏 银川 750004;2.宁夏回族自治区医学科学研究所, 宁夏 银川 750004;3.宁夏森淼枸杞科技开发有限公司,宁夏 银川 750000;4.宁夏医科大学总医院心脑血管病医院,宁夏 银川 750000;5.宁夏医科大学总医院,宁夏 银川 750004)

作者简介:

赵嘉庆 男 副教授 研究方向为感染免疫 E-mail:nxmczhaojq@163.com

通讯作者:

高小平 男 副教授 研究方向为过敏性疾病 E-mail:monsieurgao@163.com

基金项目:

宁夏回族自治区科技支撑项目(2015BY033);宁夏自然科学基金项目(NZ17159,2018AAC03142)


Mechanism of Lycium barbarum polysaccharides relieving inflammation in asthmatic mice
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(1.Basic Medical College of Ningxia Medical University, Ningxia Yinchuan 750004, China;2.Ningxia Hui Autonomous Region Medical Research Institute, Ningxia Yinchuan 750004, China;3.Ningxia Senmiao Goji Technology and Development Co. Ltd., Ningxia Yinchuan 750000, China;4.Cardiovascular Hospital, General Hospital of Ningxia Medical University, Ningxia Yinchuan 750000, China;5.General Hospital of Ningxia Medical University, Ningxia Yinchuan 750004, China)

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    摘要:

    目的 探究枸杞果多糖对Ⅴ级卵清蛋白(OVA)诱导的哮喘小鼠模型炎症损伤影响及其作用机制。方法 以OVA诱导的哮喘小鼠为模型,并进行枸杞果多糖干预。肺组织苏木素-伊红(HE)染色、流式细胞术检测肺组织嗜酸性粒细胞水平反映炎症细胞浸润程度;肺组织马松(masson)染色检测肺组织纤维化程度,过碘酸-雪夫(PAS)染色检测支气管杯状细胞化生程度;流式细胞术检测小鼠肺组织辅助性T细胞(Th1、Th2、Th17细胞)和调节性T细胞(Treg细胞)水平。结果 与对照组比较,枸杞果多糖干预的哮喘小鼠肺组织嗜酸性粒细胞水平明显降低,差异有统计学意义(P<0.05);纤维化程度明显减轻,支气管化生的杯状细胞明显减少;Th1和Treg细胞水平上调,Th2和Th17细胞水平下调,差异均有统计学意义(P<0.05)。结论 枸杞果多糖可通过调节哮喘小鼠体内的Th细胞失衡缓解气道炎症。

    Abstract:

    Objective To observe the protective effects of Lycium barbarum polysaccharides (LBPs) on lung inflammatory injury in OVA(albumin chicken egg)induced asthma mice and explore its mechanism. Methods OVA-induced allergic asthma mice model was established and mice were then treated with Lycium barabrum polysaccharides hematoxylin-eosin(HE) staining was used to detect lung tissue structure and inflammatory cell infiltration. Periodic acid-schiff(PAS) staining was used to examine bronchial goblet cell. Masson staining were used to identify collagen deposition in lung tissues. The expression of eosinophils cells, helper T cells(Th1 cells, Th2 cells, Th17 cells) and regulatory T cells(Treg cells) in lung tissue of mice was detected by flow cytometry. Results Compared with asthma mice group, after the intervention of Lycium barbarum polysaccharides, the level of eosinophils in lung tissue of asthma mice was significantly decreased(P<0.05), the degree of fibrosis was significantly alleviated, the metaplasia of bronchial goblet cells was significantly decreased, the levels of Th1 and Treg were up-regulated(P<0.05), and the levels of Th2 and Th17 were down-regulated(P<0.05). Conclusion Lycium barbarum polysaccharide extracted from Lycium barbarum fruit ameliorates asthma in mice by reducing inflammation and modulating the balance of Th cells.

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赵嘉庆,史春丽,王立英,尹梅,郑彩霞,武瑜,赵巍,高小平.枸杞果多糖对哮喘小鼠炎症损伤影响及作用机制[J].中国食品卫生杂志,2021,33(1):23-28.

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  • 收稿日期:2020-12-17
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  • 在线发布日期: 2021-04-06