北京市顺义区市售生鲜整鸡中弓形杆菌流行状况及耐药特征分析
作者:
作者单位:

1.北京市顺义区疾病预防控制中心,北京 101320;2.北京市顺义区疾病预防控制中心微生物感染性疾病检测工作站,北京 101320;3.中国疾病预防控制中心传染病预防控制所,北京 102206

作者简介:

王园园 女 主管技师 研究方向为病原微生物检测 E-mail:415809126@qq.com

通讯作者:

张茂俊 女 研究员 研究方向为感染性疾病病原学研究及诊断 E-mail:zhangmaojun@icdc.cn

中图分类号:

R155

基金项目:


Prevalence and antibiotics susceptibility of Arcobacter isolated from commercial fresh whole chicken in Shunyi District, Beijing
Author:
Affiliation:

1.Shunyi District Center for Disease Control and Prevention, Beijing 101320, China;2.Workstation for Microbial Infectious Disease, Shunyi District Center for Disease Control and Prevention, Beijing 101320, China;3.National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

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    摘要:

    目的 了解顺义区市售生鲜整鸡中弓形杆菌的污染状况及耐药特征。方法 在顺义区农贸市场、超市随机采集零售生鲜整鸡样品60份,应用滤膜法对样品进行弓形杆菌的分离培养,采用基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)及实时荧光定量PCR进行菌株鉴定,并将菌株的质谱图进行聚类分析。采用琼脂稀释法对弓形杆菌分离株进行11种抗生素的耐药性检测。结果 60份市售生鲜整鸡样品中弓形杆菌的检出率为26.67%(16/60),且均为布氏弓形杆菌。6月、7月弓形杆菌的检出率明显高于5月。16株弓形杆菌分离株经MALDI-TOF MS聚类,以相对距离1 000为节点分为两个簇群,相对距离与菌株相似性呈负相关,聚类图对样品地域来源有一定的提示作用。弓形杆菌分离株对喹诺酮类抗生素耐药严重,对萘啶酸和环丙沙星的耐药率分别为81.25%和43.75%;对庆大霉素(12.50%)、红霉素(12.50%)、阿奇霉素(12.50%)、泰利霉素(12.50%)、链霉素(6.25%)耐药率相对较低。结论 顺义区零售生鲜整鸡中弓形杆菌的检出率较高,应警惕因未烧熟煮透和交叉污染导致食源性疾病的风险。

    Abstract:

    Objective To understand the contamination status and antibiotics resistance of Arcobacter isolated from commercial fresh whole chicken in Shunyi District.Methods Sixty fresh whole chicken from retail markets and supermarkets in Shunyi District were randomly purchased. The Bacterial isolation was carried out using the membrane filtration methods. The strains were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometery and real-time fluorescent PCR. The mass spectra of the strains were clustered. The isolated Arcobacter strains were subjected to a susceptibility test for 11 antibiotics using agar dilution method.Results The detection rate of Arcobacter in 60 samples of commercially available fresh whole chicken was 26.67% (16/60) and all of them were Arcobacter butzleri. The detection rate of Arcobacter in June and July was significantly higher than that in May. Sixteen strains of Arcobacter were clustered by time-of-flight mass spectrometry and were divided into two clusters with a relative distance of 1 000. The relative distance was negatively correlated with the similarity of the strains, and the clustering map had a certain suggestive effect on the regional origin of the samples. The Arcobacter isolates were highly resistant to quinolone antibiotics and the resistance rates to nalidixic acid and ciprofloxacin were 81.25% and 43.75%, respectively. The resistance rate to gentamicin (12.50%) and erythromycin (12.50%), azithromycin (12.50%), telithromycin (12.50%) and streptomycin (6.25%) was relatively low.Conclusion The detection rate of Arcobacter in raw chickens in Shunyi District is high. It should be alert to the risk of food-borne diseases caused by undercooked cooking and cross-contamination.

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王园园,李颖,杨杰,张彦春,张爽,张赫,王苗,马红梅,张茂俊.北京市顺义区市售生鲜整鸡中弓形杆菌流行状况及耐药特征分析[J].中国食品卫生杂志,2022,34(2):297-301.

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  • 收稿日期:2021-08-25
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  • 在线发布日期: 2022-05-18
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