Objective To analyze a suspected poisoning event of edible wild vegetables by laboratory analysis， and provide scientific basis for the treatment of poisoning event and the treatment of patients.Methods The samples were qualitatively and quantitatively determined for atropine， scopolamine and anisodamine by liquid chromatography-triple quadrupole tandem mass spectrometry （LC-MS/MS）. Morphological identification was carried out on the suspicious plants collected on site， and the poisoning factors were determined by epidemiological investigation.Results On the day of poisoning， tropane alkaloids， such as atropine， scopolamine and anisodamine， were detected in the wild vegetable cake and biological samples provided by the hospital. The poisonous plant was identified as Datura stramonium L using morphological analysis. The clinical symptoms and incubation period of the patient were similar to the poisoning performance of Datura stramonium L.Conclusion The food poisoning was caused by Datura stramonium L， the laboratory test results played a key role in the rapid and accurate identification of the poisoning event.