| 张敏,卓勤,宫照龙,朴建华,杨晓光.转GmDREB1基因抗旱小麦对大鼠肠道菌群的影响[J].中国食品卫生杂志,2016,28(6):695-702. |
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| 转GmDREB1基因抗旱小麦对大鼠肠道菌群的影响 |
| Effect of genetically modified wheat with GmDREB1 gene on intestinal microflora in SD rats |
| 投稿时间：2016-11-02 |
| DOI： |
| 中文关键词: 转基因小麦 GmDREB1基因 肠道菌群 变性梯度凝胶电泳 食品安全 转基因食品 |
| Key Words:Genetically modified wheat GmDREB1 gene intestinal microflora denaturing gradient gel electrophoresis food safety transgenic food |
| 基金项目:转基因生物新品种培育重大专项(No.2011ZX08011-005,No.2014ZX08011-005) |
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| 中文摘要: |
| 通过动物试验观察转GmDREB1基因抗旱小麦对大鼠肠道菌群的细菌种类、数量的影响。方法 60只SD大鼠按性别、体质量随机分为3组:转基因小麦组(A组)、亲本小麦对照组(B组)和AIN-93对照组(C组),分别饲喂相应饲料12个月。无菌采集大鼠粪便样品,用于五种常见菌群的分离培养计数及变性梯度凝胶电泳(DGGE)分析。结果 喂养饲料后,A组及B组雄性、雌性大鼠粪便中双歧杆菌、乳杆菌的数量均高于C组,差异有统计学意义(P<0.05)；试验前后,A组及B组大鼠粪便中常见菌群数差异无统计学意义(P>0.05)；A组与B组DGGE图谱的每条泳道的条带数目、多样性指数差异无统计学意义(P>0.05)。聚类分析结果显示,A组及B组样品的组间相似性较高,未出现明显分支。结论 未发现转GmDREB1基因小麦对大鼠肠道菌群有不利影响。 |
| Abstract: |
| To observe the effect of the genetically modified wheat harboring GmDREB1 gene on intestinal microflora in sprague dawley (SD) rats.Methods Based on gender and weight, sixty SD rats were randomly divided into three groups:genetically modified (GM) wheat group (group A), non-GM wheat group (group B) and AIN-93 control group (group C). They were fed for 12 months. Feces samples were collected during the experiment period. Culture of fecal flora and denaturing gradient gel electrophoresis were used to investigate the bacterial profiles of the three groups. Results Compared with pre-experiment, the number of Bifidobacterium and Lactobacillus of group A and B increased significantly, which was significantly higher than those of group C (P<0.05). There were no significant differences between group A and group B in the number of the five feces flora, bands and Shannon's index (P>0.05). UPGMA cluster analysis indicated that group A and group B showed a high degree of similarity.Conclusion It was concluded that the genetically modified wheat with GmDREB1 gene had no detrimental effects on the intestinal microflora in SD rats. |
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