张敏,卓勤,宫照龙,朴建华,杨晓光.转GmDREB1基因抗旱小麦对大鼠肠道菌群的影响[J].中国食品卫生杂志,2016,28(6):695-702. 本文二维码信息
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转GmDREB1基因抗旱小麦对大鼠肠道菌群的影响
Effect of genetically modified wheat with GmDREB1 gene on intestinal microflora in SD rats
投稿时间:2016-11-02  
DOI:
中文关键词:  转基因小麦  GmDREB1基因  肠道菌群  变性梯度凝胶电泳  食品安全  转基因食品
Key Words:Genetically modified wheat  GmDREB1 gene  intestinal microflora  denaturing gradient gel electrophoresis  food safety  transgenic food
基金项目:转基因生物新品种培育重大专项(No.2011ZX08011-005,No.2014ZX08011-005)
作者单位E-mail
张敏 中国疾病预防控制中心营养与健康所 国家卫生计生委微量元素重点实验室,北京 100050;北京市石景山区食品药品监督管理局,北京 100043 sitong1998@126.com,gongzhaolong@163.com 
卓勤 中国疾病预防控制中心营养与健康所 国家卫生计生委微量元素重点实验室,北京 100050  
宫照龙 中国疾病预防控制中心营养与健康所 国家卫生计生委微量元素重点实验室,北京 100050 sitong1998@126.com,gongzhaolong@163.com 
朴建华 中国疾病预防控制中心营养与健康所 国家卫生计生委微量元素重点实验室,北京 100050  
杨晓光 中国疾病预防控制中心营养与健康所 国家卫生计生委微量元素重点实验室,北京 100050  
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中文摘要:
      通过动物试验观察转GmDREB1基因抗旱小麦对大鼠肠道菌群的细菌种类、数量的影响。方法 60只SD大鼠按性别、体质量随机分为3组:转基因小麦组(A组)、亲本小麦对照组(B组)和AIN-93对照组(C组),分别饲喂相应饲料12个月。无菌采集大鼠粪便样品,用于五种常见菌群的分离培养计数及变性梯度凝胶电泳(DGGE)分析。结果 喂养饲料后,A组及B组雄性、雌性大鼠粪便中双歧杆菌、乳杆菌的数量均高于C组,差异有统计学意义(P<0.05);试验前后,A组及B组大鼠粪便中常见菌群数差异无统计学意义(P>0.05);A组与B组DGGE图谱的每条泳道的条带数目、多样性指数差异无统计学意义(P>0.05)。聚类分析结果显示,A组及B组样品的组间相似性较高,未出现明显分支。结论 未发现转GmDREB1基因小麦对大鼠肠道菌群有不利影响。
Abstract:
      To observe the effect of the genetically modified wheat harboring GmDREB1 gene on intestinal microflora in sprague dawley (SD) rats.Methods Based on gender and weight, sixty SD rats were randomly divided into three groups:genetically modified (GM) wheat group (group A), non-GM wheat group (group B) and AIN-93 control group (group C). They were fed for 12 months. Feces samples were collected during the experiment period. Culture of fecal flora and denaturing gradient gel electrophoresis were used to investigate the bacterial profiles of the three groups. Results Compared with pre-experiment, the number of Bifidobacterium and Lactobacillus of group A and B increased significantly, which was significantly higher than those of group C (P<0.05). There were no significant differences between group A and group B in the number of the five feces flora, bands and Shannon's index (P>0.05). UPGMA cluster analysis indicated that group A and group B showed a high degree of similarity.Conclusion It was concluded that the genetically modified wheat with GmDREB1 gene had no detrimental effects on the intestinal microflora in SD rats.
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