沈玄艺,宋启发,高红,章丹阳,罗学辉,张建群.宁波市河水环境中沙门菌分布特征和耐药性[J].中国食品卫生杂志,2016,28(6):709-713. 本文二维码信息
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宁波市河水环境中沙门菌分布特征和耐药性
Distribution of Salmonella species and their antimicrobial resistance in a river
投稿时间:2016-08-25  
DOI:
中文关键词:  河水  沙门菌  血清型  耐药  耐药基因  宁波  食源性致病菌
Key Words:Aquatict  Salmonella species  serovar  antimicrobial resistance  resistance gene  Ningbo  foodborne pathogens
基金项目:浙江省医药卫生科技项目资助(2016KYB274);宁波市社会发展重大(重点)项目(2013c51014)
作者单位E-mail
沈玄艺 浙江省宁波市疾病预防控制中心,浙江 宁波 315010 shenxy@nbcdc.org.cn,songqf@nbcdc.org.cn 
宋启发 浙江省宁波市疾病预防控制中心,浙江 宁波 315010 shenxy@nbcdc.org.cn,songqf@nbcdc.org.cn 
高红 浙江省宁波市疾病预防控制中心,浙江 宁波 315010  
章丹阳 浙江省宁波市疾病预防控制中心,浙江 宁波 315010  
罗学辉 浙江省余姚市疾病预防控制中心,浙江 余姚 315400  
张建群 浙江省余姚市疾病预防控制中心,浙江 余姚 315400  
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中文摘要:
      了解宁波市人工河水环境中沙门菌的血清型分布特征及耐药情况,为预防、控制相应食源性疾病发生提供科学根据。方法 对河水样品进行离心浓缩、蛋白胨水增菌液增菌、亚硒酸盐胱氨酸增菌液选择性增菌和沙门菌显色平板分离沙门菌,对分离菌株采用系统生化和血清凝集进行鉴定,并采用纸片法检测菌株对8种抗生素的耐药性,对多重耐药菌株和耐β-内酰胺类抗生素菌株扩增主要相关耐药基因。结果 2014年1月至2015年12月,共采集48份河水样品,分离沙门菌株105株,主要血清型为鼠伤寒(26株)、德尔卑(14株)、里森(8株)、肠炎(4株)、阿贡纳(4株)、斯坦利(4株)、阿格玛(4株),其他及未分型沙门菌41株。70株(66.7%)耐至少一种抗生素,对抗生素的耐药率分别为氨苄西林53.3%、四环素42.9%、甲氧苄啶-磺胺甲恶唑32.4%、庆大霉素11.4%、环丙沙星4.8%、头孢噻肟4.8%和产超广谱β-内酰胺酶4.8%。β-内酰胺类抗生素相关耐药基因为TEM型(n=52)、OXA型(n=6)和CTX-M型(n=3),58.1%的菌株1类整合子基因阳性。结论 人工河水环境中沙门菌检出率较高,且部分血清型为重要的食源性致病菌,检出菌也有较高的耐药性,携带多种耐药基因,提示食源性疾病防控中需关注环境细菌的危害。
Abstract:
      The aim of this study was to explore the distribution of Salmonella species in a man-made river and their antimicrobial resistance and provide knowledge for the prevention and control of foodborne diseases.Methods River water samples were processed including centrifugation concentration, enrichment with buffered peptone water, selective cultivation with selenite-cystine broth and chromogenic plate. Antimicrobial susceptibility testing was performed using disc diffusion method. β-lactam resistance genes and intI1 gene were tested using PCR. Results From January 2014 to October 2015, a total of 105 Salmonella isolates of 12 different serovars were recovered from 48 river water samples. The most frequent isolated serovars were typhimurium (n=26), Derby (n=14), Rissen (n=8), enteritidis (n=4), Agona (n=4), Stanley (n=4) and Agama (n=4). Other 41 strains were other serovars and untypables. Overall, about 66.7% strains were resistant to at least 1 antimicrobial drug. The antimicrobial resistance rates were as following:ampicillin (53.3%), tetracycline (42.9%), trimethoprim-sulfamethoxazole (32.4%), gentamicin (11.4%), ciprofloxacin (4.8%), cefotaxime (4.8%), and extended-spectrum β-lactamase (4.8%). Four types of β-lactam resistance genes were detected, including TEM (n=52), OXA (n=6) and CTX-M (n=3). Up to 58.1% (n=61) of 105 strains were positive for class-1 integron.Conclusion High occurrence of Salmonella contamination and high level of antimicrobial resistance were detected in a man-made river. It was noteworthy that some serovars were important foodborne pathogens. These strains showed high level of antimicrobial resistance and carried several resistance genes. This situation called for future attention about the risk caused by the widespread of resistant Salmonella species in aquatic environment.
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