一起疑似食源性诺如病毒胃肠炎事件病原分子生物学特征分析
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(厦门市思明区疾病预防控制中心,福建 厦门 361009)

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王坤明 男 主管技师 研究方向为病原微生物检验E-mail:619352369@qq.com通信作者:┣┣(中)通信作者┫┫柯明月 女 主任技师 研究方向为卫生检验E-mail:kmy1973@163.com

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Pathogenic molecular biological characteristics analysis of a suspected foodborne Norovirus gastroenteritis incident
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(Siming District Center for Disease Control and Prevention of Xiamen,Fujian Xiamen 361009,China)

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    摘要:

    研究厦门市思明区一起聚集性胃肠炎事件的诺如病毒的分子生物学特征。方法 将收集到的11份肛拭子标本及1份生蚝样品,采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测诺如病毒核酸,阳性标本再进行实时荧光定量RT-PCR扩增,扩增产物经过凝胶电泳分析后,进行序列测定,确定基因型,并进行系统发育树分析。结果 11份肛拭子标本中检出5株GI组诺如病毒株,2株测序成功,检出4株GII组毒株,3株测序成功;1份生蚝样品中检出1株GI组毒株,测序成功。对测序成功的6株毒株进行同源性分析,GI.2型毒株所测序列与2014年上海株KP325648等6株参考株高度同源,GII.17型毒株所测序列与2015年韩国株KT384078等8株参考株高度同源,证实这是一起由GI.2和GII.17型诺如病毒混合感染引起的聚集性胃肠炎事件,GII.17型毒株为厦门市首次报道。结论 此次聚集性胃肠炎事件是由诺如病毒引起,且为GI.2与GII.17型毒株混合感染引起。

    Abstract:

    To analyze the molecular biological characteristics of Norovirus from a gastroenteritis incident in Siming District.Methods Eleven anal swab specimens and one oyster samples were collected and fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) method was applied to detect Norovirus. The positive samples were amplified by RT-PCR, and amplified products were used for gel electrophoresis analysis and sequencing to determine the genotype and to make phylogenetic trees analysis. Results Two strains of GI Norovirus were detected from 11 anal swab specimens, and both were successfully sequenced. Four strains of GII were detected, and 3 strains were successfully sequenced. One strain of GI was detected from the oyster, and was successfully sequenced. Six positive strains successfully sequenced were further homology analyzed. The sequence of the GI.2 strain were highly homologous with the reference strains of KP325648 etc. collected from Shanghai in 2014. The sequence of GII.17 strain were highly homologous with the reference strains of KT384078 etc. collected from South Korea in 2015. GII.17 strain was reported for the first time in Xiamen.Conclusion The aggregated gastroenteritis incident was caused by mixed infection of GI.2 and GII.17 Norovirus.

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王坤明,柯明月,施红,林英华.一起疑似食源性诺如病毒胃肠炎事件病原分子生物学特征分析[J].中国食品卫生杂志,2017,29(1):42-46.

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  • 收稿日期:2016-10-20
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  • 在线发布日期: 2017-03-15
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