高效液相色谱法测定保健食品中红景天苷
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(浙江省疾病预防控制中心,浙江 杭州 310051)

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谭莹 主管技师 研究方向为食品、保健食品分析检测E-mail:465859939@qq.com

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浙江省医药卫生科技计划平台骨干项目(2013RCA009)


Determination of salidroside in health food by high performance liquid chromatography
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(Zhejiang Center for Disease Control and Prevention,Zhejiang Hangzhou 310051,China)

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    摘要:

    改进保健食品中红景天苷的高效液相色谱(HPLC)测定方法。方法 用不同溶剂稀释对照品及提取样品,以HPLC法测定红景天苷含量,选择最佳溶剂,优化色谱条件,并作方法学论证。结果 以50%甲醇作为对照品的稀释溶液和样品的提取溶剂,红景天苷在1.305~130.5 μg/ml的线性范围内线性良好,回归方程y=7.377395×10-5x+0.1873961(r=0.999 9),最低检出浓度为0.015 mg/g;平均回收率为92.1%~98.4%,RSD为1.98%~3.26%。结论 本方法操作简便、定量准确可靠。

    Abstract:

    To improve the high performance liquid chromatography method for quantitation of salidroside in health food.Methods The solvent and chromatography condition were optimized according to the determination of standard and extracted samples. Results Salidroside content exhibited a linear relation within 1.305-130.5 μg/ml using 50% methanol as solvent. The regression equation was y=7.377395×10-5x+0.1873961 (r=0.999 9) and the detection limit was 0.015 mg/g. Furthermore, the average recoveries were 92.1%-98.4%, and the RSD was 1.98%-3.26%. Conclusion This method was simple, accurate and reliable for determination of salidroside in health food.

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谭莹,倪竹南,胡争艳,张晶,王志园,汤鋆.高效液相色谱法测定保健食品中红景天苷[J].中国食品卫生杂志,2017,29(2):164-167.

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  • 收稿日期:2016-12-16
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  • 在线发布日期: 2017-05-03
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