牛莉娅,秦丽云,徐保红,王燕,杨新英,孙殿兴,郭玉梅.2011—2016年石家庄市40株食物中毒沙门菌的耐药特征及分子分型结果分析[J].中国食品卫生杂志,2017,29(5):539-543. 本文二维码信息
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2011—2016年石家庄市40株食物中毒沙门菌的耐药特征及分子分型结果分析
Characterization of antimicrobial resistances and molecular characteristics of 40 strains of Salmonella from food poisoning in Shijiazhuang in 2011-2016
投稿时间:2017-05-15  
DOI:
中文关键词:  食物中毒  沙门菌  血清型  耐药  脉冲场凝胶电泳  石家庄
Key Words:Food poisoning  Salmonella  serotype  drug resistance  pulsed field electrophoresis  Shijiazhuang
基金项目:河北省卫生计生委2017年度医学科学研究重点课题(20170964)
作者单位E-mail
牛莉娅 白求恩国际和平医院传染肝病科,河北 石家庄 050082 83040073@qq.com,guokexin2199@163.com 
秦丽云 石家庄市疾病预防控制中心微生物检验所,河北 石家庄 050011  
徐保红 石家庄市疾病预防控制中心微生物检验所,河北 石家庄 050011  
王燕 白求恩国际和平医院传染肝病科,河北 石家庄 050082  
杨新英 白求恩国际和平医院传染肝病科,河北 石家庄 050082  
孙殿兴 白求恩国际和平医院传染肝病科,河北 石家庄 050082  
郭玉梅 石家庄市疾病预防控制中心微生物检验所,河北 石家庄 050011 83040073@qq.com,guokexin2199@163.com 
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中文摘要:
      目的 研究食物中毒沙门菌分离株的分子生物学特征,结合耐药表型,分析菌株间的同源性及耐药关系,为预防控制食源性疾病发生和临床治疗提供科学依据。方法 对分离自2011—2016年石家庄市食物中毒食品或标本的40株沙门菌进行药敏检测,并采用脉冲场凝胶电泳(PFGE)进行分子分型,运用BioNumeries软件分析菌株之间的流行病学关系。结果 40株食源性沙门菌经Xba I酶切可分为11种带型,药敏结果呈现多重耐药趋势。结论 石家庄市食源性沙门菌呈高度散发、多重耐药的特点,特别是优势血清型肠炎沙门菌耐药性较明显,提示应加强沙门菌耐药性监测。
Abstract:
      Objective To study the molecular biological characteristics of food poisoning Salmonella isolates, and analyze the homology and resistance relationship between strains, which could provide scientific basis for prevention and control of foodborne disease and clinical treatment. Methods Totally 40 strains of Salmonella isolates were tested for susceptibility and molecular typing by pulsed field electrophoresis (PFGE). BioNumeries software was used to analyze the epidemiological relationships between strains. Results All the strains could be divided into 11 types by Xba I enzyme digestion, and drug sensitivity showed multiple drug resistance. Conclusion Foodborne Salmonella was highly sporadic and multidrug resistant was found in this region. The resistance of Salmonella was obvious, especially predominance serum enteritis, which suggested that it was necessary to strengthen the surveillance of Salmonella resistance.
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