分散固相萃取-分散液液微萃取-气相色谱-质谱联用法测定中草药类保健食品中29种有机磷农药残留
作者:
作者单位:

(1.中国疾病预防控制中心营养与健康所 卫计委微量元素营养重点实验室,北京 100050;2.中国环境科学研究院 环境基准与风险评估国家重点实验室,北京 100012;3.中国环境科学研究院环境标准研究所,北京 100012)

作者简介:

马妍 女 助理研究员 研究方向为营养与食品安全E-mail:mayan_556@163.com通信作者:┣┣(中)通信作者┫┫李琴 女 研究员 研究方向为环境与健康E-mail:liqin529luck@163.com

通讯作者:

中图分类号:

基金项目:

国家自然科学基金青年科学基金资助项目(31400097)


Determination of 29 organophosphorus pesticide residues in health food from Chinese herbal medicines by dispersive solid phase extraction and dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry
Author:
Affiliation:

(1.Key Laboratory of Trace Element Nutrition,National Institute for Nutrition and Health China CDC, Beijing 100050,China;2.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences,Beijing 100012,China;3.Environmental Standards Institute,Chinese Research Academy of Environmental Sciences,Beijing 100012,China)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 建立测定中草药类保健食品中有机磷农药残留的分散固相萃取-分散液液微萃取-气相色谱-质谱联用法。方法 样品经含0.5%甲酸的乙腈超声提取,上清液经含无水硫酸镁、N-丙基乙二胺(PSA)、C18的分散固相萃取管(d-SPE)净化后,采用分散液液微萃取(DLLME)步骤,用氯仿萃取、浓缩目标化合物。结果 在最优条件下,线性范围为0.05~1.00 μg/ml,线性相关系数在0.999以上,检出限(LOD)为0.3~3.0 μg/kg,29种有机磷农药的平均加标回收率在70.3%~107.5%之间,相对标准偏差均<10%。结论 该方法具有简便快速、准确灵敏、萃取效率高等特点,可用于中草药类保健食品中有机磷农药残留的检测。

    Abstract:

    Objective To establish a method for the determination of pesticide residues in health food made from Chinese herbal medicines by dispersive solid phase extraction and dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry. Methods The samples were ultrasonic extracted by acetonitrile containing 0.5% formic acid. The supernatant was purified by dispersive solid phase extraction (d-SPE) extraction tube with anhydrous magnesium sulfate, N-propyl ethylenediamine (PSA) and C18, and then the target compounds were extracted and concentrated by the method of dispersive liquid-liquid microextraction (DLLME). Results Under optimal conditions, the linear range was 0.05-1.00 μg/ml, the linear correlation coefficient was above 0.999, the limit of detection was 0.3-3.0 μg/kg, the average recoveries of 29 kinds of pesticides were between 70.3% and 107.5%, and the relative standard deviation was less than 10%. Conclusion The method was simple, rapid, accurate and sensitive, and it could be used for the detection of organophosphorus pesticide residues in health food made from Chinese herbal medicines.

    参考文献
    相似文献
    引证文献
引用本文

马妍,陈頔,陈曦,刘婷婷,李琴.分散固相萃取-分散液液微萃取-气相色谱-质谱联用法测定中草药类保健食品中29种有机磷农药残留[J].中国食品卫生杂志,2017,29(5):556-560.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-06-23
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-10-27
  • 出版日期:
《中国食品卫生杂志》邮寄地址与联系方式变更通知
关闭