马建敏,史晓娟,邱正勇,魏红霞,王伟丽,栾旭波,李家珂.新乡市生乳中菌落总数和金黄色葡萄球菌及β-内酰胺酶调查[J].中国食品卫生杂志,2017,29(5):596-599. 本文二维码信息
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新乡市生乳中菌落总数和金黄色葡萄球菌及β-内酰胺酶调查
Investigation of aerobic plate count, Staphylococcus aureus and β-lactamas in raw milk in Xinxiang City
投稿时间:2017-07-25  
DOI:
中文关键词:  生乳  菌落总数  金黄色葡萄球菌  β-内酰胺酶  食源性致病菌  食品卫生  标准限量值  食品安全标准
Key Words:Raw milk  aerobic plate count  Staphylococcus aureus  β-lactama  foodborne pathogens  food hygiene  standard limited value  food safety standards
基金项目:
作者单位E-mail
马建敏 新乡市疾病预防控制中心,河南 新乡 453002 majianmin690@126.com 
史晓娟 新乡市疾病预防控制中心,河南 新乡 453002  
邱正勇 河南省疾病预防控制中心,河南 郑州 450016  
魏红霞 新乡市疾病预防控制中心,河南 新乡 453002  
王伟丽 新乡市疾病预防控制中心,河南 新乡 453002  
栾旭波 新乡市疾病预防控制中心,河南 新乡 453002  
李家珂 新乡市疾病预防控制中心,河南 新乡 453002  
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中文摘要:
      目的 了解新乡市规模化奶牛养殖场生乳中菌落总数、金黄色葡萄球菌和β-内酰胺酶的基线数据,为生乳安全性风险评估及国家标准更新提供资料。方法 以新乡市3家规模化奶牛养殖场(A、B、C场)为观测基地,2016年5月—2017年4月每月采集2家生乳样品各29份和1份混合样品。分别按照 GB 4789.2—2010《食品安全国家标准 食品微生物学检验 菌落总数测定》和GB 4789.10—2010《食品安全国家标准 食品微生物学检验 金黄色葡萄球菌检验》测定菌落总数和金黄色葡萄球菌,胶体金法测β-内酰胺酶残留。结果 3家奶牛养殖场单份样品菌落总数的中位数分别为13 000、4 450、130 000 CFU/ml,明显低于GB 19301—2010《食品安全国家标准 生乳》中的限量(2×106CFU/ml),差异有统计学意义(P<0.01),3家奶牛养殖场的菌落总数之间差异有统计学意义(P<0.05)。各奶牛养殖场单份样品的菌落总数与混合样品比较,仅B场差异有统计学意义(P<0.05)。7~8月菌落总数较高。3家奶牛养殖场样品中金黄色葡萄球菌检出率分别为1.1%(4/360)、16.7%(30/180)和0.0%(0/180),计数结果为50~42 000 CFU/ml。3家奶牛养殖场的所有样品中均检出β-内酰胺酶,检出率为6.1%~10.6%。结论 GB 19301—2010生乳菌落总数限量远高于新乡市实际情况,建议修订标准;个别奶牛养殖场生乳中存在一定程度的金黄色葡萄球菌污染,需加强监管;筛查出的β-内酰胺酶需进一步鉴定其来源,同时要加强对β-内酰胺酶检测方法的研究。
Abstract:
      Objective To investigate the current situation of aerobic plate count, Staphylococcus aureus and β-lactamas in raw milk in Xinxiang. Methods Twenty-nine samples and 1 mixed sample of raw milk from 2 of 3 large-scale diary farms were separately collected once a month lasting for a year. The aerobic plate count and S.aureus were determined according to the current national food safety standard, meanwhile β-lactamas was identified by colloidal gold method. Results The median of aerobic plate count (CFU/ml) of raw milk from 3 farms was 13 000,4 450 and 130 000 respectively, all significantly lower than the limit of 2×106CFU/ml in GB 19301-2010 (P<0.01). Significant difference existed between the 3 farms(P<0.05). When comparing with the mixed sample, the significant difference was only found in one farm (P<0.05). The aerobic plate count changed seasonally which was higher in July and August. S.aureus contamination rates of the 3 farms were 1.1%(4/360), 16.7%(30/180)and 0.0%(0/180), respectively, with the range of 50-42 000 CFU/ml. β-lactamas could be detected in raw milk from all three farms, and the positive rate was 6.1%-10.6%. Conclusion The limit of aerobic plate count of raw milk in GB 19301-2010 was much higher than the actual level in Xinxiang. It is recommended to revise the standard limit value. There was a certain degree of S.aureus contamination in raw milk in one of the farms, and supervision should be strengthened. Furthermore, identification of the source of β-lactamas was needed, the research on the detection method of β-lactamase should also be strengthened.
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