椰毒假单胞菌酵米面亚种选择性分离培养基的比较研究
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(1.四川省疾病预防控制中心,四川 成都 610041;2.云南省疾病预防控制中心,云南 昆明 650034)

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黄伟峰 男 主管技师 研究方向为微生物检验 E-mail:hyc0608@163.com通信作者:┣┣(中)通信作者┫┫杨小蓉 女 主任技师 研究方向为微生物检验 E-mail:yangyangxr@163.com

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Comparative study of selective culture medium of Pseudomonas cocovenenans subsp. farinofermentans
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(1.Sichuan Province Center for Disease Control and Prevention, Sichuan Chengdu 610041, China;2.Yunnan Province Center for Disease Control and Prevention, Yunnan Kunming 650034, China)

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    摘要:

    目的 比较4种椰毒假单胞酵米面亚种选择性分离培养基(马铃薯葡萄糖琼脂,PDA;改良马铃薯葡萄糖琼脂,mPDA;椰毒假单胞菌酵米面亚种分离琼脂,PCFA;银耳卵黄氯霉素琼脂,TYCA)的分离效果,为修订GB/T 4789.29—2003《食品卫生微生物学检验 椰毒假单胞菌酵米面亚种检验》提供技术支持。方法 接种椰毒假单胞菌酵米面亚种于4种选择性分离培养基,观察各培养基上目标菌菌落形态,计算生长率;接种10种常见致病菌和环境中常见菌于4种选择性分离培养基,进行生长特异性试验;通过直接涂布和增菌划线的加标试验,验证这4种选择性分离培养基对粪便、食品和环境土壤等不同标本/样品中椰毒假单胞菌酵米面亚种的分离检出情况。结果 mPDA和PCFA能够分别抑制8种和6种致病菌的生长,且椰毒假单胞菌酵米面亚种的生长率都高于75%;在mPDA和PCFA上,目标菌与多数杂菌形态有明显区别,而在PDA和TYCA上,形态相近不易区分;在粪便、土壤和食品等不同标本/样品的直接涂布和增菌划线分离的加标试验中,mPDA和PCFA上的检出率均超过80%,明显高于PDA和TYCA。结论 mPDA和PCFA分离效果比原标准的PDA明显提高,建议在标准修订中增加此两种选择性分离培养基。

    Abstract:

    Objective To study and compare the separation effect of four types of selective culture media for Pseudomonas cocovenenans subsp. farinofermentans, including potato dextrose agar (PDA), modified potato dextrose agar (mPDA), Pseudomonas cocovenenans subsp. farinofermentans agar (PCFA) and tremella yolk chloramphenicol agar (TYCA), and provide technical support for the national standard GB/T 4789.29-2003. Methods Pseudomonas cocovenenans subsp. farinofermentans was inoculated on four types of media, the colony morphology and the growth rate were observed. Specificity test of growth status was carried out on four types of media by inoculating ten types of pathogenic bacteria and common environmental bacteria. The separation effect of four types of media was verified in different standard strain-added samples, including stool food and soil, by directly coating and enrichment streaking. Results The growth of 8 and 6 pathogenic bacteria could be inhibited respectively on mPDA and PCFA, and the growth rate of Pseudomonas cocovenenans subsp. farinofermentans could reach above 75%.The target bacteria in mPDA and PCFA had showed apparent difference compared with most of the interfering bacteria; while the difference of their colonies was not significant in PDA medium and TYCA. In the standard strain-added experiments, both directly coating and enrichment streaking of different samples such as stool, soil and food, the detection rate of mPDA and PCFA was above 80%,and was significantly higher than that of PDA and TYCA. Conclusion The separation effect of mPDA and PCFA was significantly better than that of PDA, and these two selective media could be added to the revised standard.

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黄伟峰,黄玉兰,杨祖顺,张林,刘丽,李莉,杨小蓉.椰毒假单胞菌酵米面亚种选择性分离培养基的比较研究[J].中国食品卫生杂志,2018,30(4):391-395.

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  • 收稿日期:2018-02-24
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  • 在线发布日期: 2018-08-16
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