2017年我国沙门菌定性检验及血清学分型质量控制考核结果研判
作者:
作者单位:

(1.国家食品安全风险评估中心 国家卫生健康委员会食品安全风险评估重点实验室, 北京 100021;2.北京农学院食品科学与工程学院,北京 102206)

作者简介:

胡豫杰 男 助理研究员 研究方向为食源性沙门菌 E-mail:huyujie@cfsa.net.cn通信作者:┣┣(中)通信作者┫┫李凤琴 女 研究员 研究方向为食品微生物 E-mail:lifengqin@cfsa.net.cn

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基金项目:

国家食品安全风险评估中心高层次人才队伍建设“523”项目


Analysis on quality control assessment results of Salmonella qualitative detection and serotyping in China, 2017
Author:
Affiliation:

(1.NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment,Beijing 100021, China;2.Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China)

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    摘要:

    目的 评估我国食品安全风险监测体系各检验机构(简称检验机构)食源性沙门菌的定性检测能力,并全面了解各级检验机构实验室沙门菌血清学分型能力概况,确保沙门菌检验结果准确可靠,提升监测质量。方法 批量制备菌球样品(含6种我国常见血清型沙门菌和阴性对照)和样品基质,经稳定性等测试后发放至435家检验机构进行考核,使用点分数法对上报结果进行评价,Pearson卡方检验进行率的比较。结果 431家检验机构上报检验结果,初测所有指标完全正确率为72.6%(313/431),总体满意率为92.6%(399/431),结果不满意的主要类别为阴性样品假阳性(7.4%,32/431),区县级检验机构满意率(80.0%,52/65)低于省级(100.0%,32/32)和地市级(94.3%,315/334)。血清学分型考核完全正确比例为80.7%(348/431),不同级别检验机构间血清群正确率差异无统计学意义(P>0.05),但地市级检验机构血清型正确率低于省级甚至区县级。结论 本次考核提示各级检验机构的沙门菌定性检验能力基本能够满足监测任务需求,但部分实验室仍需要加强质量控制以提升沙门菌检验水平,省级以下实验室血清型鉴定水平仍需进一步提高,本次质量控制考核为开展针对性的监测培训提供了数据支持。

    Abstract:

    Objective A quality control assessment was conducted to assess the Salmonella qualitative detection capability of responsible unit in inspection institutions of food safety risk monitoring system in China, and to fully understand Salmonella serotyping ability of monitoring laboratories at different level, in order to guarantee reliable detection data and isolates, and enhance the monitoring quality. Methods Bacterial samples including 6 different common Salmonella serovars and negative control were prepared with substrate samples, and distributed to 435 participates after stability test. The result were evaluated with point-score system and ratios were analyzed with Pearson χ2 test. Results A total of 431 reports were received from the participate laboratories, 72.6% (313/431) of which were all correct, and the overall satisfaction rate was 92.6% (399/431). The main category for the dissatisfaction was the false positive of the negative samples(7.4%,32/431). The satisfaction rate of county participates(80.0%,52/65)was lower than provincial(100.0%,32/32)and prefecture level(94.3%,315/334). The correct rate of serotyping section was 80.7% (348/431). There was no significant difference in the correct rate of serogroups between different levels (P>0.05), but the correct rate of serotypes in prefecture-level laboratories was lower than that of provincial or even county level participates. Conclusion This assessment indicates that ability of Salmonella qualitative detection at all levels of task-undertaking units in inspection institutions can basically meet the requirements, however, some laboratories need to improve quality control in order to strengthen ability of Salmonella detection. A continuing need for improvement on serotype identification still existed among laboratories below provincial level. This quality control assessment could play a role in providing a data basis for training in inspection institutions.

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胡豫杰,刘畅,徐进,王美美,李志刚,余东敏,赵熙,张宏元,韩春卉,张靖,李燕俊,李业鹏,裴晓燕,李宁,李凤琴.2017年我国沙门菌定性检验及血清学分型质量控制考核结果研判[J].中国食品卫生杂志,2018,30(6):615-621.

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  • 收稿日期:2018-10-28
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  • 在线发布日期: 2019-01-23
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