菠萝蜜低聚肽对60钴γ射线辐射损伤小鼠防护作用
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(北京大学公共卫生学院,北京 100191)

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郝云涛 男 硕士生 研究方向为生物活性肽与健康E-mail:herbertlly@163.com通信作者:┣┣(中)通信作者┫┫李勇 男 教授 研究方向为营养与疾病E-mail:liyongbmu@163.com

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Radioprotective effect of jackfruit oligopeptides in 60Co γ ray-irradiated mice
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(School of Public Health, Peking University, Beijing 100191, China)

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    摘要:

    目的 探究菠萝蜜低聚肽(JOPs)的辐射防护作用。方法 采用无特定病原体(SPF级)雌性BALB/c小鼠,按体质量随机分为空白对照组、模型对照组、乳清蛋白组(0.40 g/kg BW)及JOPs低、中、高剂量组(0.20、0.40、0.80 g/kg BW),每组24只,共144只;每组随机分为3个亚组,每个亚组8只。灌胃干预第14天,除空白对照组外,其他小鼠接受60钴γ射线全身辐射,辐射后继续灌胃干预。各组第1亚组(辐射剂量8 Gy,剂量率1 Gy/min)进行30 d存活试验,另外2个亚组(辐射剂量3.5 Gy,剂量率1 Gy/min)分别在辐射后第3天和第14天检测外周血白细胞(WBC)数、骨髓细胞DNA含量、体质量变化及肝脏、脾脏与胸腺的脏器指数。结果 与模型对照组比较,JOPs中、高剂量组小鼠生存时间明显延长,差异有统计学意义(P<0.05);辐射后第3天和第14天,JOPs各剂量组小鼠WBC数、骨髓细胞DNA含量均明显升高,肝脏、脾脏指数明显增加,差异均有统计学意义(P<0.05);辐射后第14天,JOPs低、中剂量组小鼠胸腺指数明显增加,差异有统计学意义(P<0.05),且JOPs的各项功效均优于乳清蛋白。结论 JOPs对电离射线辐射损伤具有防护作用。

    Abstract:

    Objective To investigate the radioprotective effect of jackfruit oligopeptides (JOPs). Methods One hundred and forty-four specific pathogen free(SPF)female BALB/c mice were randomly divided into blank control group, model control group, whey protein group (0.40 g/kg BW) and 3 JOPs intervention groups (0.20,0.40,0.80 g/kg BW) with 24 in each group. Each group was randomly divided into 3 subgroups, 8 per subgroup. On the 14th day of the intragastric administration, the mice except the blank control group received 60Co γ-ray whole body irradiation, and intragastric intervention continued after irradiation. The first subgroup of each group (8 Gy, 1 Gy/min) was subjected to 30 d survival test, and the other two subgroups (3.5 Gy, 1 Gy/min) were used to detect peripheral blood white blood cells (WBC), bone marrow DNA content, weight changes and organ indexes of liver, spleen and thymus on the 3rd and 14th day after irradiation, respectively. Results Compared with the model control group, the survival time of the medium and high dose group of JOPs was significantly prolonged(P<0.05). On the 3rd and 14th day after irradiation, the WBC number, the DNA content of bone marrow cells, the liver and spleen indexes in the JOPs intervention groups were significantly increased(P<0.05). Besides, the thymus index of the low and middle dose groups of JOPs increased significantly on the 14th day after irradiation(P<0.05). Furthermore, the efficacy of JOPs was superior to those of whey protein. Conclusion JOPs had protective effects against ionizing radiation exposure.

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郝云涛,珠娜,刘睿,刘欣然,康家伟,胡佳妮,毛瑞雪,刘思奇,张亭,张晓玄,李勇.菠萝蜜低聚肽对60钴γ射线辐射损伤小鼠防护作用[J].中国食品卫生杂志,2019,31(4):325-329.

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  • 收稿日期:2019-07-02
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  • 在线发布日期: 2019-09-16
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