樊华,胡静,李雨哲,于洲,周宇红,徐海滨,梁春来.纳米氧化锌28 d经口毒性及其对肠道免疫影响研究[J].中国食品卫生杂志,2019,31(5):415-422. 本文二维码信息
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纳米氧化锌28 d经口毒性及其对肠道免疫影响研究
Study on 28-day oral toxicity of nano zinc oxide and its effect on intestinal immunity
投稿时间:2019-07-01  
DOI:
中文关键词:  纳米氧化锌  经口毒性  肠道免疫
Key Words:Zinc oxide nanoparticles  oral toxicity test  intestinal immunity
基金项目:“十三五”国家重点研发计划(2016YFD0400601)
作者单位E-mail
樊华 宁夏医科大学,宁夏 银川 750004 1031721501@qq.com,liangchunlai@cfsa.net.cn 
胡静 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021  
李雨哲 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021  
于洲 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021  
周宇红 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021  
徐海滨 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021  
梁春来 国家食品安全风险评估中心 国家卫生健康 委员会食品安全风险评估重点实验室,北京 100021 1031721501@qq.com,liangchunlai@cfsa.net.cn 
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中文摘要:
      目的 研究短期经口摄入纳米氧化锌对大鼠的毒性作用及其对肠道免疫的影响。方法 将断乳SD大鼠随机分为4组(对照组和低、中、高剂量组),每组雌、雄各10只,连续28 d灌胃给予纳米氧化锌,剂量分别为87.5、175、350 mg/kg BW,观察体质量及摄食量变化;试验结束后禁食取血,检测各项血液学、血生化指标,并剖检进行脏器称重以及组织病理学检查;取肠道派氏结进行淋巴细胞分型检测以及开展肠液中分泌型免疫球蛋白A(SIgA)检测。结果 大鼠一般状况正常,体质量、进食量、脏器重量、脏体比以及血常规、凝血、血生化等指标均未发现有意义改变;组织病理学检查发现,部分动物出现胃黏膜局灶性上皮细胞脱落,黏膜下层以嗜酸性粒细胞为主的炎细胞浸润及水肿,小肠绒毛上皮脱落,与对照组比较,高剂量组病变发生例数增加;肠道派氏结淋巴细胞分型检测显示,雄性大鼠中、高剂量组T细胞比例增高,自然杀伤(NK)细胞比例降低,各剂量组SIgA浓度差异无统计学意义(P>0.05)。结论 短期经口摄入纳米氧化锌,在350 mg/kg BW剂量条件下,可导致大鼠胃肠黏膜损伤,并对肠道免疫指标产生影响。
Abstract:
      Objective To study the short-term oral toxicity and the effect of zinc oxide nanoparticles on intestinal immune in rats. Methods Weaning rats were randomly divided into 4 groups:control group, low, medium and high-dose groups (10 male and famale rats in each group). Zinc oxide nanoparticles were intragastrically administered at doses of 87.5,5 and 350 mg/kg BW for 28 days, respectively. Blood samples were collected on day 29 for measurement of hematology and clinical biochemistry. Animals were euthanized for necropsy, and selected organs were weighed and fixed for histological examination. Flow cytometric analysis of lymphocyte subsets of Peyer's patch and the level of secretory immunoglobulin A (SIgA) in intestinal fluid were detected. Results There were no toxicologically significant changes in clinical signs, body weight, food consumption, necropsy findings and organ weights, hematological and clinical biochemical values. Histopathological examination showed an increased incidence of focal epithelial cell exfoliation in gastric mucosa, inflammatory cell infiltration and edema in gastric submucosa, and villous epithelial cell exfoliation in small intestine. Compared with the control group, the number of lesions in the high dose group increased significantly. Lymphocytes phenotyping analysis showed a significant increase in percentage of T lymphocytes and decrease in percentage of natural killer(NK)cell in Peyer's patch. The concentration of SIgA had no significant difference between dose groups(P>0.05). Conclusion The ingestion of zinc oxide nanoparticles can cause gastrointestinal mucosal damage and can affect the intestinal immune index in rats, at 350 mg/kg BW dose.
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