雷高鹏,黄玉兰,吕虹,黄伟峰,杨小蓉.四川省鸭和猪源鼠伤寒沙门菌脉冲场凝胶电泳分型与耐药比较分析[J].中国食品卫生杂志,2019,31(5):441-444. 本文二维码信息
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四川省鸭和猪源鼠伤寒沙门菌脉冲场凝胶电泳分型与耐药比较分析
Comparative analysis of antimicrobial resistance and pulsed-field gel electrophoresis typing of Salmonella Typhimurium isolated from duck and swine in Sichuan Province
投稿时间:2019-09-10  
DOI:
中文关键词:  鼠伤寒沙门菌  脉冲场凝胶电泳分型  细菌耐药  食源性致病菌
Key Words:Salmonella Typhimurium  pulsed field gel electrophoresis typing  antimicrobial resistance  foodborne pathogens
基金项目:四川省卫生和计划生育科研课题(16PJ395)
作者单位E-mail
雷高鹏 四川省疾病预防控制中心,四川 成都 610041 lionleonleo@126.com,yangyangxr@163.com 
黄玉兰 四川省疾病预防控制中心,四川 成都 610041  
吕虹 四川省疾病预防控制中心,四川 成都 610041  
黄伟峰 四川省疾病预防控制中心,四川 成都 610041  
杨小蓉 四川省疾病预防控制中心,四川 成都 610041 lionleonleo@126.com,yangyangxr@163.com 
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中文摘要:
      目的 比较研究四川省鸭和猪源鼠伤寒沙门菌脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型与耐药特征,为分析鼠伤寒沙门菌食品来源提供支持。方法 将鸭和猪源监测中分离的47株鼠伤寒沙门菌进行PFGE分型,并采用最小抑菌浓度法测定9种药物敏感性。结果 鸭和猪源中分离的鼠伤寒沙门菌在PFGE分型和耐药上存在差异。鸭源菌株聚集于簇I,而猪源菌株聚集于簇II。簇II菌株对氨苄西林、环丙沙星、氯霉素、庆大霉素、四环素、复方新诺明6种药物的耐药率高于簇I菌株。不同的PFGE分型鼠伤寒沙门菌在监测地区和耐药谱存在差异。SCSTm-10型菌株均分离于绵阳地区,SCSTm-11型主要分离于资阳地区。SCSTm-10型菌株对复方新诺明和环丙沙星的耐药率高于SCSTm-11型菌株。结论 PFGE分型可以获取四川省鼠伤寒沙门菌分离来源、分离地点和耐药的聚类信息,为鼠伤寒沙门菌食物中毒提供流行病学调查支持。
Abstract:
      Objective To study the pulsed field gel electrophoresis(PFGE)type and antimicrobial resistance of Salmonella Typhimurium (S. Typhimurium) isolated from duck and swine, so as to provide support for source-tracking of S.Typhimurium strains in Sichuan Province. Methods A Total of 47 S. Typhimurium strains isolated from pigs and ducks were analyzed by PFGE and tested for antimicrobial resistance for 9 antibiotics by minimal inhibitory concentration (MIC) method. Results S. Typhimurium strains isolated from pigs and ducks differed in PFGE type and antimicrobial resistance. The strains isolated from ducks were clustered in cluster I and the pig source strains clustered in cluster II. The resistance rate of strains from pigs to the 6 kinds of antibiotics including ampicillin, ciprofloxacin, chloramphenicol, gentamycin, tetracycline and sulfamethoxazole compound trimethoprim were significantly higher than that of strains from ducks. S.Typhimurium with different PFGE types were different in the isolation areas and antimicrobial resistance. SCSTm-10 strains were located in MY region, and SCSTm-11 were mainly isolated in ZY region. The resistant rates of SCSTm-10 on sulfamethoxazole/trimethoprim and ciprofloxacin were higher than SCSTm-11. Conclusion PFGE typing could obtain the clustering information of isolated source, isolation location and antimicrobial resistance of S.Typhimurium in Sichuan Province, and provide support for the epidemiological investigation of S. Typhimurium infections.
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