食品中2株非常见耶尔森菌分离鉴定及耐药性分析
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(上海市疾病预防控制中心,上海 200336)

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罗嘉远 男 技师 研究方向为耶尔森菌属检测E-mail:luojiayuan@scdc.sh.cn 陈敏 男 主任技师 研究方向为细菌学检测E-mail:chenmin@scdc.sh.cn

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Isolation and drug resistance of 2 stains of Yersinia from food
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(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

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    摘要:

    目的 对食品中两株Yersinia aleksiciae(Ya)进行分离鉴定及耐药性分析研究,为非常见耶尔森菌属菌株的分离鉴定提供技术储备。方法 2018年8月至2019年3月采集猪肉、牛肉、鸡肉食品样品,共300份。对分离到的疑似Ya菌株完成生化鉴定、耐药表型鉴定和全基因组测序分析。结果 食品样品中Ya的检出率为0.7%(2/300),生化鉴定中Ya与小肠结肠炎耶尔森菌(Yersinia enterocolitica,Ye)最大区别是Ye为赖氨酸脱羧酶阴性,Ya为赖氨酸脱羧酶阳性。Ya暂未发现与Ye类似的对氨苄西林、I代头孢菌素、替卡西林等抗生素的天然耐药性,两株Ya菌株的耐药性不同。全基因组测序比对16S rRNA和gyrB、rpoD基因三个片段序列,证实两株分离菌株为Ya。结论 分离到的两株Ya为国内首次发现,目前国外鲜有从食品中分离出该菌的报道。本研究提供的方法可用于其他非常见耶尔森菌的分离鉴定。

    Abstract:

    Objective Identification and drug resistance of two stains of Yersinia aleksiciae (Ya) isolated from different foods were studied, which provided technical reserves for the isolation of the new Yersinia spp. Methods A total of 300 food samples were collected from August 2018 to March 2019, including pork, beef and chicken. Separation culture, biochemical identification, drug resistance test, and whole genome sequencing analysis were performed on the isolated suspected Ya. Results The positive rate of Ya in food samples was 0.7% (2/300). In biochemical identification, Ya was distinguished from Yersinia enterocolitica (Ye) by lysine decarboxylase test, and Ya was positive for lysine decarboxylase while Ye was negative. Ya didn't exhibit natural resistance to ampicillin, cephalosporin I, ticarcillin and other drugs similar to Ye, and the drug resistance was different between the two strains. 16S rRNA, gyrB, rpoD genes were compared by whole genome sequencing to confirm that the two isolates were Ya. Conclusion The two strains of Ya were isolated and found in China for the first time. There was also no report on the isolation of these strains from food. The method provided in this study could be used for the isolation and identification of new Yersinia spp.

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罗嘉远,陈洪友,陈敏,窦剑明,高芬,张曦.食品中2株非常见耶尔森菌分离鉴定及耐药性分析[J].中国食品卫生杂志,2020,32(1):20-24.

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  • 收稿日期:2019-12-25
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  • 在线发布日期: 2020-02-25
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