Objective To develop a real-time reverse transcription polymerase chain reaction (RT-PCR) method for detection of Norovirus GⅡ (NoV GⅡ) in water. To survey NoV GⅡ in 10 water samples. MethodsNitrocellulose membrane and PEG precipitation were used to enrich virus in bottle water, river and sewage samples, and real-time RT-PCR method was developed. The recoveries from MS2 spiked samples were used to evaluate the effect of the established method. Results The average recoveries of bottle water, river and sewage samples were (60.1±8.0)%, (22.0±6.5)% and (35.7±8.1)% respectively. Three in 10 submitted water samples were positive for NoV GⅡ. Conclusion A real-time PCR method for detection of NoV GⅡ in water sources was developed in this study. NoV G Ⅱ was one of the reasons of the poisoning incidents.