食品营养强化剂L-苏糖酸镁中L-苏糖酸含量高效液相色谱检测方法的建立及优化

2025,37(2):138-144
田洪芸，王勇，张颖，刘毅，陆垣宏，胡明燕，胡梅，王骏
山东省食品药品检验研究院，山东省特殊医学用途配方食品质量控制工程技术研究中心，国家市场监管重点实验室（肉及肉制品监管技术），产业技术基础公共服务平台，山东 济南 250101
摘要：目的 建立基于高效液相色谱检测食品营养强化剂L-苏糖酸镁产品中L-苏糖酸含量的方法。方法 试样经水溶解后，使用C18反相色谱柱分离，以0.1%磷酸水溶液-乙腈为流动相，流速为0.8 mL/min，柱温为40 ℃，使用二极管检测器于210 nm下进行检测。结果 L-苏糖酸标准溶液在20~500 μg/mL范围内线性关系良好，相关系数r2>0.999，检出限为1 g/100 g，定量限为2.5 g/100 g。日内精密度、日间精密度测试结果显示相对标准偏差为0.10%~0.19%，再现性测试结果显示测试结果相对标准偏差为0.25%~0.40%。结论 该方法精密度高、稳定性好、操作简便，可满足L-苏糖酸镁产品质量控制的要求。
关键词：L-苏糖酸镁;L-苏糖酸;液相色谱法;检出限;定量限;精密度

Establishment and optimization of a HPLC method for determination of L-threonic acid in magnesium L-threonate

TIAN Hongyun, WANG Yong, ZHANG Ying, LIU Yi, LU Yuanhong, HU Mingyan, HU Mei, WANG Jun
Shandong Institute of Food and Drug Control， Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes， Key Laboratory of Supervising Technology for Meat and Meat Products for State Market Regulation， Industrial Technology Foundation Public Service Platform, Shandong Ji’nan 250101， China
Abstract:Objective To develop a high-performance liquid chromatography-based method for the determination of L-threonate in magnesium L-threonate products.Methods The samples were dissolved in water and separated by C18 reversed-phase chromatography with 0.1% phosphoric acid solution and acetonitrile as mobile phase. The flow rate was 0.8 mL/min and the column temperature was 40 ℃， detection was performed at 210 nm using a diode detector.Results The linear relationship between the reference materials of L-threonoid acid was good in the range of 20-500 μg/mL， and the correlation coefficient was r2>0.999. The limit of detection was 1 g/100 g， and the limit of quantification was 2.5 g/100 g. The relative standard deviation （RSD） of intra-day precision and inter-day precision was 0.10%-0.19%， and RSD of the reproducibility was 0.25%-0.40%.Conclusion The method has the advantages of high precision， good stability and simple operation， and can meet the requirements of quality control of magnesium L-threonate product.
Key words:Magnesium L-threonate;L-threonic acid;chromatography;detection limit;qnantitative limit;precision
投稿时间：2024-08-06
基金项目：国家卫生健康管理委员会食品安全国家标准制定项目（spaq-2019-118）；山东省市场监督管理局科技创新项目（2022298）
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