青岛市牡蛎养殖场副溶血性弧菌污染水平及时间分布
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(青岛市疾病预防控制中心 青岛市预防医学研究院,山东 青岛 266033)

作者简介:

刘晓琳 女 副主任技师 研究方向为微生物检验E-mail:chxlxl@sina.com

通讯作者:

于维森 男 主任技师 研究方向为卫生检验E-mail:yuweisen@126.com

基金项目:

青岛市民生科技计划项目(18-6-1-67-nsh)


Contamination level and temporal variation of Vibrio parahaemolyticus in oyster farm in Qingdao
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(Qingdao Center for Disease Control and Prevention, Qingdao Institute of Preventive Medicine, Shandong Qingdao 266033, China)

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    摘要:

    目的 了解青岛市牡蛎养殖场副溶血性弧菌污染水平及在不同的季节随时间和温度变化的趋势,为食品安全风险评估提供参考。方法 在2013年6月—2014年5月采用最大可能实时荧光定量聚合酶链式反应(the most probable number real-time polymerase chain reaction,MPN real-time PCR)方法检测牡蛎养殖场环境及牡蛎中副溶血性弧菌的污染水平,并分析与时间和温度的相关性。结果 牡蛎和海水中不耐热溶血毒素(thermolabile hemolysin,tlh)基因MPN最低值出现在1~3月份,6月份之前呈缓慢上升趋势,之后快速上升,至8月份达到最高峰,之后快速下降,1月份降至最低,海水在1~3月份未检出tlh基因。海泥中tlh基因MPN均值自5~12月份一直维持在高峰水平(18 067.5~24 000.0 MPN/g),1月份下降明显,至3月份降至最低(88.0 MPN/g)。结论 与传统方法比较,MPN real-time PCR法敏感性好,能灵敏地反映副溶血性弧菌随时间温度变化的生长趋势,准确评估牡蛎和环境中的带菌水平,进而发挥预警作用。青岛市牡蛎养殖场海水和牡蛎中副溶血性弧菌的污染水平在6~8月份较高,在加工食用过程中应该注意,避免引起食源性疾病。

    Abstract:

    Objective To investigate temporal variation in the abundance of total Vibrio parahaemolyticus in shellfish farm in Qingdao. Methods The abundance of total V.parahaemolyticus in shellfish and farm environment was detected using the most probable number real-time polymerase chain reaction(MPN real-time PCR) method during June 2013 and May 2014, and the correlation between V.parahaemolyticus abundance and the season was analyzed. Results The lowest level of MPN in shellfish and seawater presented from January to March,increased slowly before June,then rapidly rised up and reached the highest level until Augest. After that,it deceased rapidly to the lowest level in January. The lowest level of MPN in sediment was in March and the highest level kept from May to December(18 067.5-24 000.0 MPN/g),then dropped rapidly in January, and reached the lowest level in March(88.0 MPN/g). Conclusion Compared with the traditional methods, MPN-PCR has better sensitivity in reflecting V.parahaemolyticus growing trend with the the season and tempreture change.The level of bactiera abundence in oyster and seawater can be accurately assessed,which might play a role of early warning.The rapid growth time of V.parahaemolyticus in seawater and oyster is from June to Augest, which should be taken notice in the process of handling and eating to avoid foodborn disease.

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刘晓琳,王慧,王丽娟,曲剑英,郭凯,于维森.青岛市牡蛎养殖场副溶血性弧菌污染水平及时间分布[J].中国食品卫生杂志,2021,33(1):19-22.

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  • 收稿日期:2020-11-23
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  • 在线发布日期: 2021-04-06