2014—2019年成都市新都区鼠伤寒沙门菌耐药特性和分子分型研究
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(1.成都市新都区疾病预防控制中心,四川 成都 610500;2.四川省疾病预防控制中心,四川 成都 610041)

作者简介:

王尔群 女 副主任技师 研究方向为微生物检验E-mail:76705725@qq.com

通讯作者:

黄伟峰 男 主管技师 研究方向为微生物检验E-mail:hyc0608@163.com

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Drug resistance characteristics and molecular typing of Salmonella Typhimurium in Xindu District, Chengdu, 2014-2019
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(1.Chengdu Xindu District Center for Disease Control and Prevention, Sichuan Chengdu 610500, China;2.Sichuan Province Center for Disease Control and Prevention, Sichuan Chengdu 610041, China)

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    摘要:

    目的 了解新都区2014—2019年分离的54株鼠伤寒沙门菌耐药情况和分子分型特征。方法 对从病例监测、食物中毒和食品监测分离的54株鼠伤寒沙门菌用微量肉汤稀释法进行药敏试验,用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)进行分子分型。结果 54株鼠伤寒沙门菌耐药率为98.15%(53/54),三重及以上耐药率为46.30%(25/54)。病例监测和食品监测菌株的多重耐药率分别为56.00%(14/25)和91.67%(11/12),远高于食物中毒菌株0.00%(0/17)。PFGE结果分为31种带型,相似度为64.9%~100%,其中优势带型为XD005,包含7株菌,均来源于病例监测和食品监测。食物中毒来源菌株与病例监测、食品监测来源菌株的耐药情况和分子分型结果差异均有统计学意义(P<0.05)。结论 新都区病例监测和食品监测来源的鼠伤寒沙门菌耐药情况较为严重。PFGE能在散发病例中识别潜在的暴发事件,应及时对分离菌株进行分子溯源。

    Abstract:

    Objective To understand the drug resistance and molecular typing characteristics of 54 strains of Salmonella Typhimurium in Xindu District from 2014 to 2019. Methods The 54 strains of Salmonella Typhimurium isolated from case monitoring, food poisoning and food surveillance were tested for drug susceptibility using micro-broth dilution method, and pulsed field gel electrophoresis (PFGE) was used for molecular typing. Results The drug resistance rate of 54 strains of Salmonella Typhimurium was 98.15%(53/54), and that of multi-drug resistance (triple and above)was 46.30%(25/54). The multi-drug resistance rates of the case monitoring and food surveillance strains were 56.00% (14/25) and 91.67% (11/12), respectively, which were much higher than the food poisoning strains 0.00% (0/17). The PFGE results could be divided into 31 pattern, with a similarity of 64.9%-100%. Among them, the dominant pattern was XD005, which contains 7 strains isolated from case monitoring and food surveillance. The results of drug resistance and molecular typing of strains from food poisoning were significantly different from those from case monitoring and food surveillance(P<0.05). Conclusion The drug resistance of Salmonella Typhimurium isolated from case monitoring and food surveillance was serious in Xindu District. PFGE can identify potential outbreak events in sporadic cases, so it is necessary to trace the molecular origin of the isolated strains on time.

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王尔群,舒玉聪,宋志琴,秦超,黄伟峰.2014—2019年成都市新都区鼠伤寒沙门菌耐药特性和分子分型研究[J].中国食品卫生杂志,2021,33(1):69-74.

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  • 收稿日期:2020-10-14
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  • 在线发布日期: 2021-04-06