Diphenoxylate was administrated to mice by the dose of 10 mg/kg BW for the development of constipation model for formulation of examination procedure of health foods. The time of defecation of the first black urine, quantity and weight of urine with 6 hours were statistically significant different compared with the control group. The results presented the stability and reliability of the model and it was recommended to verify in the function experiments of health foods.