In order to examine the effect of grape seed extract procyanidin on blood lipid regulation, healthy adult male SD rats were fed with lipid-rich diet for 6 weeks. Meanwhile, procyanidins at three different concentrations of 25 mg/kg BW, 100 mg/kg BW and 150 mg/kg BW were given to rat by gavage daily throughout the 6 weeks. The levels of serum TC, TG, and HDL-C, liver TC, and TG and the activities of serum LCAT, as well as the excretion of fecal TAB were measured. The results showed that procyanidins decreased the levels of serum TC by 18^1% and TG by over 34^4% (P<0^05) in rats fed with lipid-rich diet, and the levels of liver TC and TG of hyperlipidemic rats (P<0.01). In addition, procyanidins significantly increased the activity of serum LCAT (P<0.05) and the excretion TBA. The results suggested that procyanidins had the effect of regulating lipid metabolism of hyperlipidemic rats.