Objective: To investigate the serotype, pathogenicity and drug resistance of Yersinia enterocolitica causing a case of foodborne disease. Method: Serum agglutination method, modified K-B paper method and PCR were used for serotyping，evaluation on sensitivity, detection of virulence genes（ail, ystB, virF）and the rfbc gene which biosynthesizing the specific lipopolysaccharide O-side chain of O:3 serotype strain. Results: The Y. enterocolitica strain isolated in this study is positive in O:3 serotyping test, carrying the virulence genes of ail and virF and specific gene rfbc but no ystB. The strain is resistant to cefazolin, ampicillin, and sulfamethoxazole compound, but sensitive to other drugs such as cephalosporins, carbapenems, and aminoglycoside. Conclusions: The case of foodborne disease is caused by pathogenic non-biotype 1A serotype O:3 Yersinia enterocolitica strains carrying virulence genes of ail and virF.