Objective To evaluate the effectiveness of a lead-expelling food on expelling mineral matters and expelling lead from lead poisoning mice induced by acetate lead. Methods Seventy-five Kuming male mice weighted 18-22 g were randomly divided into five groups: negative control group, lead poisoning model group and three intervention groups fed with low, medium and high dosage of lead-expelling food. Deionized water was provided for the negative control group and 1.00g/L lead acetate solution was provided for the lead-poisoning model group and three intervention groups freely. The lead-expelling food for the low, medium and high dose intervention groups was administrated intragastrically with 5-, 10- or 20-fold of the recommended dosage for human use (0.60g/kgBW). The general situation and body weight changes were observed in 30 days. The content of lead, calcium, iron, zinc and copper in blood, liver, femur, kidney and brain were determined. Results The lead content of liver, kidney and brain in low, medium and high-dose intervention groups were lower than those in the lead-poisoning model group (P＜0.05 or P＜0.01). The lead content of blood and femur in medium and high-dose intervention group were lower than those in lead-poisoning model group (P＜0.05 or P＜0.01). The calcium and iron of liver, zinc of brain, copper of kidney in low-, medium- and high-dose intervention group were higher than those in the lead-poisoning model group (P＜0.05or P＜0.01). The calcium of blood, kidney and brain, the iron, zinc and copper of blood, the blood and copper of brain in middle- and high-dose intervention groups were higher than those in lead-poisoning model group (P＜0.05 or P＜0.01). Iron of femur in high-dose intervention group was higher than that in lead-poisoning model group (P＜0.05). Conclusion The lead-expelling food plays a role in promoting lead elimination from mice, and can improve calcium, iron, zinc and copper metabolism disorders in lead poisoning mice significantly.