Objective To establish a method for the determination of 10 sulfonamides in honey, including sulfanilamide, sulfadiazine, sulfapyridine, sulfamerazine, sulfamethazine, sulfameter, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole and sulfisoxazole, with matrix solid phase dispersion and high performance liquid chromatography. Methods Honey sample was grinded and well-distributed with a solid-phase sorbent C₁₈ in a glass mortar to form a semi-solid mixture. The mixture was packed as a solid phase extraction (SPE) cartridge, covered with a piece of paper on the top of the mixture to produce a sample/column material; and then eluted with 20ml 1+3 methylene-ethyl acetate (V/V). The eluted materials were collected and evaporated to dry at 40℃ by nitrogen gas. The residues were dissolved in 0.5ml of mobile phase solvent and passed through a 0.45μm filter membrane, and then were separated by an Agilent TC-C₁₈ column (4.6mm×250mm，5μm) and determined by HPLC. Results The linear ranges of 10 sulfonamides were 0.10-10.0 mg/L, r>0.999. The recovery ranges were 60.0%-109.3% and RSD<10%. The detection limits were 0.016-0.025mg/L and the quantification limits were 0.050~0.079mg/kg.