Abstract:Objective To establish a simple, sensitive and accurate method for the determination of ten kinds of synthetic colorant in food at the same time. Methods Synthetic colorants, including Tartrazine, New Red, Amaranth, Indigo Blue, Ponceau 4R, Sunset Yellow, Allura Red, Brillinat Blue, Quinoline Yellow and Erythrosine B in food samples were prepared with the procedures described in GB/T 5009.35 and were extracted by polyamide adsorption. Sample solutions were separated by reverse phase liquid chromatography with carbinol/acetonitrile (3+1) and acetic ammonium solution (0.02mol/L,pH=4) mixture as mobile phase under gradient elution, and quantified by DAD (Photodiode Array Detector). Results The calibration curves showed a good linearity with correlation coefficients from 0.9990 to 1.The linear range for the quantification of Tartrazine, Amaranth, Indigo Blue, Ponceau 4R and Sunset Yellow was 0.94-30.00μg/ml, New Red was 0.94-30.00μg/ml, Allura Red was 1.00-32.10μg/ml, Brillinat Blue was 0.31-10.00μg/ml, Quinoline Yellow was 1.21-9.68μg/ml and Erythrosine was 0.76-24.4μg/ml. The detection limit was from 0.03 mg/kg to 0.9mg/kg. The recovery of the method was from 87.5% to 101.4%,and the relative standard deviation (RSD) was from 1.3% to 5.7%. Conclusion The method is sensitive, simple and accurate, and can be used to quantify these synthetic colorant in foods at the same time. The method could meet the requirement for fast and accurate detection of synthetic colorants in food.