Objective To synthesize and identify artificial antigens for pyrethroids. Methods Two haptens were synthesized by using Kung Fu chrysanthemic acid and 3-Phenoxybenzoicacid as the intermediate of pyrethroids to react with a four carbon “spacer arm”—aminobutyric acid, which were verified by MS. Immunogens for immunizing Balb/c mouse were prepared by the N-hydroxysuccinimide-activated ester method using bovine serum albumin as carriers; and coating antigens were prepared by mixed anhydride reaction using ovalbumin as carriers. The concentration of conjugates and molar ratios were estimated by UV spectrophotometry. Results The titer of polyclonal antisera determined by ELISA was high (>1:50000) and the IC50 was 200 ng/ml. Conclusion The artificial antigens for pyrethroids were prepared successfully.
