Objective To understand the molecular epidemiology traits of Vibrio parahaemolyticus in Jiangsu and surrounding areas.Methods Biochemical identification,tdh and trh gene detection,serological typing and pulse-field gel electrophoresis(PFGE) were applied to 63 Vibrio parahaemolyticus strains isolated from food poisoning incidents occurred in Jiangsu and surrounding areas in 2010.Results The positive rates of tdh and trh were 79.4%(50/63) and 1.6%(1/63) respectively.The main serological type of strains isolated from patients was O3∶K6(26/57),and that from food was O1∶KUT(4/6).The patterns of PFGE showed that the similarity of groups from A to E was more than 60%.Conclusion Strains isolated from different regions in Jiangsu and surrounding areas were in high homology.The food poisoning incidents occurred in these areas might be contaminated by Vibrio parahaemolyticus strains from the same source.