Objective To investigate the contamination of Enterohemorrhagic escherichia coli(EHEC) O157∶ H7 on food in Wenzhou,and to understand its virulent factors and drug resistance.Methods Food samples were collected by random in 2008-2011 and were isolated for EHEC O157∶ H7 by IMS.The contamination of isolates was identified by biochemical methods and serum subtyping and the drug susceptibility was tested by K-B method;O157 and H7 antigen gene and the virulence genes of stx1,stx2,eaeA and hlyA were detected by PCR.Results A EHEC O157∶ H7 strain was isolated from 231 food samples(0.43%);in which the O157 and H7 antigen gene was positive,but the virulence genes were not detected,and the strain was resistant to erythromycin,rifampin at concentration of 150 μg and 10 μg of 0/129.Conclusion The contamination of EHEC O157∶ H7 was existed in food in Wenzhou,although the detection rate was low.It suggests that strengthening active surveillance through effective intervention to prevent EHEC O157∶ H7 caused by food-borne diseases is necessary.