Abstract： Objective YT-1107 is a Leclercia adecarboxylata strain isolated from contaminated food. The study of physiological and biochemical characteristics and 16S rRNA sequencing was aimed to identify its species. Methods Suspected pathogens were selected according to epidemiological survey and clinical manifestations, and then samples were isolated and identified by ATB Biolog microbial identification system. 16S rRNA gene sequencing results were analyzed for homology and MEGA4.0 Neighbor-Joining method was used to construct phylogenetic tree. Result Based on the physiological and biochemical characteristics and 16S rRNA sequencing, the strains were gram negative bacteria, belonging to Enterobacter asburiae. Considering the evidence of homology comparison of 16S rRNA gene sequencing and phylogenetic tree construction, this strain was preliminary identified as the same speciesas Enterobacter asburiae LF7a. Conclusion Strain YT-1107 and Enterobacter asburiae LF7a are the same species.