Aflatoxins (AFTs), a group of toxic secondary metabolites, are produced by species of Aspergilli, especially Aspergillus flavus and Aspergillus parasiticus. AFTs have been proved to be potent carcinogens, mutagens, and steratogens. The contamination of AFTs in cereal and foods brings a significant health problem due to their strong toxicity. Developing antiaflatoxigenic inhibitors has become a research hotspot. Some antibiotics and chemical fungicides were effective Aspergilli inhibitor, but were not widely used because of their obvious toxic and adverse effects. In recent two decades, some natural antiaflatoxigenic inhibitors with lower side effects and easy degradation have been discovered. This paper summarized reports about anti-aspergilliand antiaflatoxigenic activities of plant essential oils, their constituents and relevant application. This information may provide reference for further research on the antimicrobial mechanisms of plants volatile oils and product development.