To establish an accurate, rapid and specific fluorescent PCR method to detect pathogenic gene of Vibrio parahaemolyticus(VP).Methods7VP standard stains were used to verify 6literary methods of fluorescent PCR and the new method establish in this study. The specificity was verified using 6common foodborne pathogenic bacteria. ResultsThe newly-established method could detect all 3kinds of hemolysins simultaneously, and the results were consistent with those of stains tracing and standard PCR methods. There was no cross reaction with common foodborne pathogens.ConclusionThe fluorescent PCR system could detect pathogenic gene of VP with better accuracy, reliability, speed and specificity, which was more applicable.