To analyze the protein components in shrimp which could bind with serum specific IgE (sIgE) of allergy patients.MethodsThe shrimp protein extracts were prepared by routine method, and protein components were separated by SDS-PAGE. Using serum of shrimp allergy patients as probe, the protein components which could bind with sIgE were identified by immunoblotting technique. ResultsThe SDS-PAGE of shrimp protein extracts showed 12identifiable bands, and the protein content of 65,0and 36kD were relatively higher than the others. Although the serum of allergy patients showed different affinity and binding sites in the immunoblotting assay, the identified protein was mainly in the region of molecular weight greater than 70kD. ConclusionWhen reacted with positive serum, the reaction intensity and protein content were unrelated. The sensitivity of detection for serum sIgE might be improved by extracting active protein components of shrimp.