武汉市部分菜场肉类食品中产志贺毒素大肠杆菌污染状况分析
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毕旺来 男 实验员 研究方向为食品安全和食品微生物E-mail:605277709@qq.com

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国家“863”计划项目(2012AA101703-3);武汉市科技局国际科技合作计划项目(2013030409020113)


Analysis on contamination status of Shiga toxin-producing Escherichia coli in retail meats from some markets in Wuhan
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    摘要:

    对武汉市部分菜场肉类食品中产志贺毒素大肠杆菌(STEC)污染状况进行分析。方法 2011年7月至2012年10月期间,从武汉市汉口30个菜场和超市共采集样品196份,其中猪肉102份,牛肉60份,禽类34份。通过选择性增菌,提取DNA,PCR扩增stx1、stx2、rfbO157、wzyO157基因,分析食品中产志贺毒素大肠杆菌的污染状况。结果 猪肉、牛肉、禽类食品中非O157型STEC的阳性检出率分别为18.6%、48.4%和2.9%;O157型STEC的阳性检出率分别为13.6%(猪肉)、6.7%(牛肉)和2.9%(禽类)。菜场样品STEC检出率(35.8%)略高于超市样品(32.4%)。结论 武汉市肉类食品中STEC检出率较高,应定期跟踪监测,了解菌株流行和毒力变化状况。

    Abstract:

    To investigate the contamination of Shiga toxin-producing Escherichia coli (STEC) in retail meats in Wuhan city.MethodsA total of 196samples were collected from 30open markets and supermarkets during a two-year sampling period (from 2011July to 2012October). Specimens comprised 102pork, 60beef and 34poultry samples. All samples were performed selective incubation, then screened by PCR amplification for the presence of stx1, stx2, rfbO157 and wzyO157 genes. ResultsShiga toxin-producing Escherichia coli (STEC) were frequently detected in beef, pork and poultry products. 18.6% raw meats, 48.4% beef and 2.9% poultry samples had been tested positive for the presence of non-O157STEC. The detection rate of O157type STEC was 13.6% in pork, 6.7% in beef and 2.9% in poultry, respectively. The detection rate of STEC in open markets (35.8%) was a little higher than that in supermarkets (32.4%).ConclusionThe STEC detection rate is a little high in retail meats in Wuhan, and it should be concerned.

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毕旺来,谈笑,陈程,程登陆,龙程钰,王宏勋,李睿.武汉市部分菜场肉类食品中产志贺毒素大肠杆菌污染状况分析[J].中国食品卫生杂志,2014,26(1):67-70.

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  • 收稿日期:2013-09-18
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  • 在线发布日期: 2014-03-04
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