To establish a multiplex PCR (mPCR) method to identity Campylobacter jejuni and Campylobacter coli.MethodsSpecific primer pairs were designed based on the sequence of 16S rRNA gene, hippuricase gene and 16S-23S rRNA gene. 37strains were detected by the mPCR and ⁱⁿgene CAM nested PCR assay kit. ResultsThe results showed that the species-specific product could be detected after amplification of the DNA template of C.jejuni and C.coli, while other strains could not be detected. The sensibility for detection of C.jejuni and C.coli was 0.81and 0.93pg/μl, respectively. The coincidence rate mPCR method and nested PCR assay kit was 100%. Coincidence of the two methods with the national standard method were also over 97%.ConclusionThis new method was rapid, convenient, highly specific, sensitive and repeatable. It could be used for rapid identification of Campylobacter spp..