To find out the contamination status and distribution of pathogenic Vibrios in aquatic products exporting to Macao.Methods A random sampling method were used to collect samples from Zhuhai and Zhongshan. Samples were preliminary screened by multiplex PCR based on HAND system and confirmed by traditional culture and biochemical identification. The contamination status and seasonal, regional and species distribution were finally analyzed. Results Compared with 37.3% coexistent infection rate, the positive rate of Vibrios in aquatic samples was 59.5%. The positive rate of pathogenic Vibrios from May to November was significantly higher than that of January and December. The overall positive rate in Zhuhai district was 69.5%, higher than that in Zhongshan (51.9%) and the difference was statistically significant (P<0.05). The overall positive rate of shellfish was 70.6%, higher than that of fish (54.4%) and the difference was statistically significant (P<0.05).Conclusion The contamination status of pathogenic Vibrios was serious in aquatic products exporting to Macao and needed to monitor to prevent infection.