To determine toxins in food poisoning and its contents fast and accurately, and to provide a scientific basis for emergency response of food poison.Methods According to the epidemiological investigation guide of food safety accidents and the diagnosis standard of WS/T 82-1996、WS/T 81-1996, routine methods and multiplex real-time PCR were used for 30 suspected samples involved vomits、diarrhea contents、food remainder by local CDC. ResultsWaxy bacillus enterotoxin was detected from vomits,and Bacillus cereus was also found in 2 food remainder,Vibrio parahaemolyticus was found in 3 diarrhea contents and 1 food remainder.Conclusion This food safety accident was caused by mixed infection with Bacillus cereus and Vibrio parahaemolyticus.