Loop-mediated isothermal amplification (LAMP) method was developed to detect fox-derived ingredients in mutton.Methods Four pairs of specific primers (F3, B3, FIP, BIP) were designed, respectively. The sensitivity and specificity test were performed after the LAMP assay was optimized. Then the detection of fox-derived ingredients in mutton was carried out.Results The optimized condition was 0.2 μmol/L each of F3 and B3, 1.6 μmol/L each of FIP and BIP, 0.5 mol/L betaine, 0.4 mmol/L dNTP and 4 mmol/L MgSO₄. 1.0% adulterated fox-derived ingredients could be detected specifically. The limit of detection was 2×10-4 ng/μl.Conclusion This method is highly specific and sensitive, and could be a very useful tool for mutton adulteration detection