A method was developed for the rapid screening of sildenafil, vardenafil and thioaildenafil in health liquor by electrospray ionization-ion mobility spectrometry(ESI-IMS).Methods The samples were extracted by ultrasound with 100% acetonitrile for 20 min and then the supernatant was diluted by 90% acetonitrile. The IMS was operated in the positive mode at ambient pressure using ESI as the ionization source and air as drift gas and the analytes were quantified by the matrix-matched external method. Results The calibration curves showed good linearity within the range of 1.25-12.50,2.00-20.00,1.00-10.00 mg/L for sildenafil, vardenafil and thioaildenafil, respectively. The detection limits(LODs) of sildenafil, vardenafil, thioaildenafil were 0.50,0.80,0.40 mg/L, respectively, and the recoveries were 93.33%-121.17% with the relative standard deviations (RSD, n=6) of 2.65%-10.00%. The results were in accordance with LC-MS/MS.Conclusion The method is simple, rapid and accurate, and can be applied as a screen method for the analysis of sildenafil, vardenafil and thioaildenafil in health care liquor.