To compare LightMix Kit Norovirus detection kit from Roche diagnostics company(hereinafter referred to as “Roche method”) with different methods of standards and norms. Methods Two hundred and twenty-seven stool samples of diarrhea patients and 158 shellfish samples from retail markets were detected by Roche method and SN/T 2626-2010 detection of Norovirus at frontier port reverse transcription polymerase chain reaction to detect Norovirus method(hereinafter referred to as SN/T method). By parallel detection, the differences between the two methods were compared. Results For stool samples, about 27.2%(59/217)stool samples were positive by Roche method, while 17.5% (38/217) samples were positive by SN/T method. The consistence between the two methods was 85.7% and the detection rate of Roche methods was significantly higher than that of SN/T method(P＜0.05). For shellfish samples, about 32.9%(52/158)shellfish samples were positives by Roche method and 30.4%(48/158)samples were positive by SN/T method. The consistence between the two methods was 87.3% and the detection rate had no significant difference(P＞0.05).Conclusion The Norovirus contamination of shellfish samples from retail market was high. Roche method was rapid, simple, sensitive and reliable to detect Norovirus, and was worthy to promote in food microbiology testing industry.