朱婧,石羽杰,吴立芳,孙赫南,王鑫,王竹,关蕾,余恺,杨月欣.不同阶段母乳中10种游离低聚糖的检测及含量分析[J].中国食品卫生杂志,2017,29(4):417-422. 本文二维码信息
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不同阶段母乳中10种游离低聚糖的检测及含量分析
Detection and comparison of 10 human milk oligosaccharides during lactational stage
投稿时间:2017-04-25  
DOI:
中文关键词:  母乳  泌乳阶段  母乳低聚糖  超高效液相色谱  检测
Key Words:Breast milk  lactational stage  human milk oligosaccharides  ultra high performance liquid chromatography  detection
基金项目:北京市科学技术研究院青年骨干计划(201527)
作者单位E-mail
朱婧 北京市营养源研究所,北京 100069 juer120@hotmail.com,yxyang@263.net 
石羽杰 雀巢研究中心北京分部,北京 100095  
吴立芳 中国疾病预防控制中心营养与健康所,北京 100050  
孙赫南 雀巢研究中心北京分部,北京 100095  
王鑫 北京中科邦尼国际科技有限责任公司,北京 100069  
王竹 中国疾病预防控制中心营养与健康所,北京 100050  
关蕾 雀巢研究中心北京分部,北京 100095  
余恺 雀巢研究中心北京分部,北京 100095  
杨月欣 中国疾病预防控制中心营养与健康所,北京 100050 juer120@hotmail.com,yxyang@263.net 
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中文摘要:
      目的 检测不同阶段母乳中10种游离母乳低聚糖(human milk oligosaccharides,HMOS)的含量,并比较其在不同阶段母乳中的变化。方法 分别采集产后0~7 d(初乳)、产后8~15 d(过渡乳)、产后16~180 d(成熟乳)的母乳,采用荧光标记HMOS,通过超高效液相色谱-荧光检测法和标准曲线定量10种HMOS。采用Person相关比较不同泌乳时间与HMOS含量的相关性,3个泌乳阶段的组间差异采用单因素方差分析。结果 在选定的色谱条件下,10种HMOS可完全分离并定量。10种HMOS中,2′岩藻糖乳糖(2′FL)、3′唾液酸乳糖(3′SL)、6′唾液酸乳糖(6′SL)、乳糖-N-四糖(LNT)、乳糖-N-新四糖(LNnT)、乳糖-N-五糖I(LNFP-I)与泌乳时间存在负相关关系,3′岩藻糖乳糖(3′FL)与泌乳时间存在正相关关系,上述7种HMOS在不同阶段母乳中差异有统计学意义(P<0.05),而α-四糖(PI)、乳糖-N-五糖V(LNFP-V)、乳糖-N-新五糖(LNnFP-V)与泌乳时间无明显相关关系,在不同阶段母乳中含量差异无统计学意义(P>0.05)。结论 不同阶段母乳中HMOS的含量不同。10种HMOS中,7种HMOS与泌乳时间具有相关关系并且在不同泌乳阶段差异有统计学意义(P<0.05)。
Abstract:
      Objective To detect 10 kinds of human milk oligosaccharides (HMOS) and compare their amounts during lactational stage. Methods Breast milk samples in different stages of lactation as colostrum (day 0-7 postpartum), transitional milk (day 8-15 postpartum), and mature milk (day 16-180 postpartum) were collected and 10 HMOS in those samples were detected and quantified by ultra high performance liquid chromatography-fluorescence detection after fluorescence labeling by using standard curves. Correlations between HMOS and lactation day were conducted by Person correlation analysis method , while the differences among three stages were calculated by ANOVA test. Results Ten HMOS were successfully separated and quantified under chosen chromatographic parameters. 2′FL,3′SL, 6′SL, LNT, LNnT and LNFP-I were negatively correlated and 3′FL was positively correlated with lactation days. They were different in three lactational stages (P<0.05), while PI, LNFP-V and LNnFP-V showed no correlation and difference (P>0.05). Conclusion The amount of HMOS changed during lactational stages. Seven HMOS were correlated with lactation days and different in three lactational stages (P<0.05).
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