Objective A method for the simultaneous determination of 14 plant growth regulator residues in cucumbers and tomatoes, etc. by QuEChERS-ultra-high performance liquid chromatography (UPLC) was developed. Methods The samples were extracted by CH₂Cl₂-CH₃CN (1∶1,V/V) containing 0.5% formic acid, and purified with dispersive solid phase extraction. Chromatographic analysis was carried out on Agilent InfinityLab Poroshell 120 EC-C₁₈ column (4.6 mm×100 mm, 2.7 μm) with acetonitrile-0.05% acetic acid as the mobile phase using gradient elution, and detected at 220 and 254 nm. Results Rapid separation of 14 analytes could be performed in 12 minutes. The correlation coefficients of calibration curves were above 0.999. The limits of detection and quantification were in the ranges of 0.005-0.076 mg/kg and 0.02-0.32 mg/kg. The average recoveries varied from 71.9% to 113.8% with relative standard deviations less than 5.0%. Conclusion The method was rapid for simultaneous determination of varied residues. It was simple,reliable and cost-effective and was suitable for quantitative screening of large quantities of samples.