Objective To investigate molecular characteristic and drug resistance patterns of Cronobacter isolated from retail infant foods. Methods Fifty strains of Cronobacter isolated from infant foods between 2010-2016 were typed by pulsed field gel electrophoresis(PFGE)after their chromosomal DNA were digested with restriction endonucleases Xba I, and the PFGE patterns were analyzed by BioNumerics software. Antibiotic susceptibility test was carried out by micro-broth dilution method to analyze drug resistance patterns. Results Fifty Cronobacter strains revealed 47 PFGE patterns with similarity ranged from 28.7% to 100.0%. Isolates from different regions or different enterprises were distributed among clusters with highly polymorphic, and without regional aggregates and specificity. But four strains isolated from different batches of two enterprises belong to the same clone. All strains were sensitive to nalidixic acid, ciprofloxacin, cefepime, amikacin, cefotaxime and ceftriaxone. The strains were resistant to sulfadiazine, ampicillin, chloramphenicol, sulfamethoxazole-trimethoprim, amoxicillin-clavulanic acid, kanamycin and gentamicin at different degrees. And 7 strains were multi-drug resistant.Conclusion It should be paid more attention to the contamination of Cronobacter in the infant foods, because some enterprises may be contaminated with sustained Cronobacter and some multiple antibiotic resistant strains appeared in the study.