Objective To prepare the standard sample for quantitative analysis of okadaic acid （OA） and dinophysistoxins-1 （DTX1） based on the mixed matrix of Pectinidae and Prorocentrum lima.Methods Pectinidae tissue and algae mud of Prorocentrum lima containing OA and DTX1 were used as raw materials. The matrix OA and DTX1 reference materials were prepared by mixing and freeze-drying. The qualitative confirmation， uniformity and stability test and collaborative constant value analysis were carried out.Results It was confirmed that the reference materials contained OA and DTX1. The homogeneity was good by variance analysis （F test）. The long-term stability and short-term stability under simulated transportation conditions were investigated， and the materials performance was stable. The characteristic standard value and uncertainty of OA and DTX1 in the reference materials were （7.039 ± 0.272） mg/kg （k = 2） and （2.012 ± 0.021） mg/kg （k = 2）， respectively.Conclusion The mixed matrix reference material can fill the absence of such reference material in China at present. It can be used for laboratory quality control such as test method verification， test process quality control and capability verification.