基于检测概率模型的表面增强拉曼光谱法快速筛查果蔬中多菌灵残留
作者:
作者单位:

1.上海海关动植物与食品检验检疫技术中心,上海 200135;2.上海如海光电科技有限公司,上海 201209

作者简介:

陈念念 女 工程师 研究方向为食品检测 E-mail: n_smily@163.com

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中图分类号:

R155

基金项目:

国家重点研发计划项目(2018YFC1603503);长三角科技合作项目(19395810100);上海市农业领域项目(19391901500)


Rapid screening of carbendazim residues in fruits and vegetables using surface enhanced Raman spectroscopy based on the model of probability of detection
Author:
Affiliation:

1.Technical Center for Animal Plant and Food Inspection and Quarantine, Shanghai Customs, Shanghai 200135, China;2.Shanghai Oceanhood Opto-eletronics Tech Co., LTD, Shanghai 201209, China

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    摘要:

    目的 建立一种水果蔬菜中多菌灵残留的表面增强拉曼光谱(SERS)快速筛查方法。方法 样品经乙醇提取、二氯甲烷净化、氮吹浓缩和乙醇溶液复溶四步获取待测液,通过单因素实验,优化金纳米粒子、提取液、萃取试剂及促凝剂的加入量,确定拉曼光谱仪的仪器测试条件。结果 多菌灵的拉曼特征位移为630±5 cm-1、728±5 cm-1、1 002±5 cm-1、1 224±5 cm-1、1 264±5 cm-1、1 316±5 cm-1,根据其拉曼光谱特征峰及其强度,对多菌灵进行定性快速测定。结合检出概率模型(POD),确定出多菌灵在苹果、柑橘等水果中的检出限为0.5 mg/kg,在辣椒等蔬菜中的检出限为1.0 mg/kg。结论 本方法操作简单、快速准确,从样品前处理到结果显示仅需40 min,结合使用POD模型,验证了该方法的有效性,可应用于水果蔬菜中多菌灵的现场快速检测。

    Abstract:

    Objective To develop a detection method for rapid screening of carbendazim residues in fruits and vegetables using surface enhanced Raman spectroscopy (SERS) based on the model of probability of detection.Methods The test solution was obtained after ethanol extraction, purification using dichloromethane, nitrogen blowing concentration and ethanol re-dissolution. The parameters including the addition amount of gold nanoparticles, extraction solution, extraction reagent and coagulant were optimized by single factor experiment, and the instrument test conditions of Raman spectrometer were determined.Results The displacement of carbendazim was 630±5 cm-1, 728±5 cm-1, 1,002±5 cm-1, 1,224±5 cm-1, 1,264±5 cm-1 and 1,316±5 cm-1. According to its Raman spectrum characteristic peak and its intensity, carbendazim was determined qualitatively and rapidly. With the probability of detection (POD), the detection limit of carbendazim in apple, citrus and other fruits was 0.5 mg/kg, and that in pepper and other vegetables was 1.0 mg/kg.Conclusion This method is simple, fast and accurate. It takes only 40 min from sample pretreatment to the results. Combined with POD model, the effectiveness of the method is verified. And the method can be used for field rapid detection of carbendazim residues in fruits and vegetables.

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陈念念,古淑青,任硕,赵超敏,邓晓军,赵志慧.基于检测概率模型的表面增强拉曼光谱法快速筛查果蔬中多菌灵残留[J].中国食品卫生杂志,2022,34(4):700-706.

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  • 收稿日期:2021-12-31
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  • 在线发布日期: 2022-08-26
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