济南市售生鸡肉中金黄色葡萄球菌污染状况及半定量风险评估研究
作者:
作者单位:

1.山东大学齐鲁医学院公共卫生学院微生物检验学系,山东省“十三五”高等学校感染性疾病防控 重点实验室,山东 济南 250012;2.国家食品安全风险评估中心,国家卫生健康委员会食品安全 风险评估重点实验室,北京 100021

作者简介:

阚浩鹏 男 在读研究生 研究方向为微生物检验 E-mail:kanhaopeng@mail.sdu.edu.cn

通讯作者:

温红玲 女 教授 研究方向为微生物检验 E-mail:wenhongling@sdu.edu.cn

中图分类号:

R155

基金项目:

国家重点研发计划(2018YFC1602703)


Study on Staphylococcus aureus contamination and semi-quantitative risk assessment in raw chicken sold in Ji’nan
Author:
Affiliation:

1.Department of Microbiological Laboratory Technology, School of Public Health, Cheeloo College of Medicine, Shandong University, Key Laboratory of Infectious Disease Control and Prevention in Universities of Shandong, Shandong Ji’nan 250012, China;2.Key Laboratory of Food Safety Assessment of Ministry of Health, China National Center for Food Safety Assessment, Beijing 100021, China

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    摘要:

    目的 了解济南市售生鸡肉中金黄色葡萄球菌污染状况和分离株肠毒素基因携带情况,评估生鸡肉产品对消费者的健康风险。方法 2021年3~11月对济南市主要人口密集区域的部分大型超市、农贸市场、肉制品零售店等流通环节及电商平台进行样品采集,依据GB 4789.10—2016《食品微生物学检验 金黄色葡萄球菌检验》要求对样品进行金黄色葡萄球菌检验;通过聚合酶链式反应(PCR)对分离株进行复核鉴定和5种肠毒素基因(seasebsecsedsee)的检测;使用Risk ranger风险评估软件评估济南市售生鸡肉中金黄色葡萄球菌对消费者健康的影响。结果 在采集的180份样品中,共55份样品检出金黄色葡萄球菌,其中大型超市、电商平台来源的冷冻鸡制品16份(16/61);农贸市场,肉制品零售店来源的冷冻鸡制品30份(30/60),鲜整鸡9份(9/59)。55株分离株中有9株携带至少一种经典肠毒素基因。半定量风险评估结果提示,济南市售生鸡肉中金黄色葡萄球菌导致消费者罹患食物中毒的风险等级为高风险水平,其中农贸市场、肉制品零售店来源的冷冻鸡制品风险值为53;鲜整鸡风险值为52;大型超市、电商平台来源的冷冻鸡制品风险值为49。结论 济南市市售生鸡肉中金黄色葡萄球菌污染水平较为严重。结合风险评估结果,建议有关部门加强监管力度,规范市售冷冻肉制品的运输和存放条件,降低冷冻鸡制品细菌污染的风险,保障消费者健康。

    Abstract:

    Objective To understand the contamination of Staphylococcus aureus in raw chicken sold in Ji’nan and the carrying of enterotoxin genes in isolated strains, and assess the health risks of raw chicken products to consumers.Methods From March 2021 to November 2021, samples were collected from e-commerce platforms and circulation links such as large supermarkets, farmers’ markets, and meat retail stores in the main densely populated areas of Ji’nan city. The samples were tested according to GB 4789.10—2016 “Food Microbiology Testing Staphylococcus Aureus Test” to identify and detect 5 enterotoxin genes (sea seb sec sed see) of isolates through polymerase chain reaction (PCR). The risk assessment software of risk ranger was used to assess the impact of Staphylococcus aureus in raw chicken sold in Ji’nan on consumer health.Results Among the 180 samples collected, a total of 55 samples detected Staphylococcus aureus, including 16 frozen chicken products from large supermarkets and e-commerce platforms (16/61), 30 frozen chicken products from farmers’ markets and meat retail stores (30/60), and 9 fresh whole chickens (9/55). A total of 9 of the 55 isolates carried at least one classical enterotoxin gene. The semi-quantitative risk assessment results suggested that the risk level of Staphylococcus aureus caused by food poisoning in raw chicken sold in Ji’nan was high risk level, of which the risk value of frozen chicken products from farmers’ markets and meat product retail stores was 53, the risk value of fresh whole chicken was 52, and the risk value of frozen chicken products from large supermarkets and e-commerce platforms was 49.Conclusion The pollution level of Staphylococcus aureus in raw chicken sold in Ji’nan is relatively serious. Based on the risk assessment results, it is recommended that relevant departments strengthen supervision, standardize the transportation and storage conditions of frozen meat products on the market, reduce the risk of bacterial contamination of frozen chicken products, and protect the health of consumers.

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阚浩鹏,李楠,胡豫杰,赵丽,刘畅,杨柱,温红玲.济南市售生鸡肉中金黄色葡萄球菌污染状况及半定量风险评估研究[J].中国食品卫生杂志,2022,34(5):968-973.

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  • 收稿日期:2022-07-18
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  • 在线发布日期: 2022-12-01
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