胶体金试纸条在豆芽喹诺酮快速检测的应用
作者:
作者单位:

1.四川省食品检验研究院,四川 成都 610000;2.国家市场监管重点实验室(白酒监管技术), 四川 成都 610000

作者简介:

刘美 女 主管药师 研究方向为食品检测与分析 E-mail:125293102@qq.com

通讯作者:

余晓琴 女 正高级工程师 研究方向为食品安全研究与分析 E-mail:113343838@qq.com

中图分类号:

R155

基金项目:


Application of colloidal gold immunochromatography on rapid detection of quinolones in bean sprouts
Author:
Affiliation:

1.Sichuan Institute of Food Inspection, Sichuan Chengdu 610000, China;2.Key Laboratory of Baijiu Supervising Technology for State Market Regulation, Sichuan Chengdu 610000, China

Fund Project:

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    摘要:

    目的 研究胶体金免疫层析法检测豆芽中诺氟沙星、氧氟沙星、双氟沙星、恩诺沙星、环丙沙星、氟罗沙星、达氟沙星、培氟沙星、洛美沙星和沙拉沙星10种喹诺酮类药物的适用性。方法 取豆芽样品2 g,经5 mL提取液提取、过滤、2倍稀释后,加入至金标微孔孵育,后与试纸条反应。通过比较检测线(T线)与控制线(C线)的颜色深浅判读结果;考察与6-苄基腺嘌呤、4-氯苯氧乙酸、甲硝唑、尿素、氯吡脲交叉反应;通过真实阳性样品和加标样品考察方法的可靠性。结果 方法检出限可达到5 μg/kg;常规检出水平条件下,6-苄基腺嘌呤、4-氯苯氧乙酸、甲硝唑、尿素以及氯吡脲等与喹诺酮类药物不发生交叉反应;真实阳性样品和多水平加标样品证实方法可靠。结论 该方法简单快速,对试剂、耗材、人员等要求不高,易于判读,结果准确可靠,适合现场快速检测。

    Abstract:

    Objective To study the applicability for determination of norfloxacin, ofloxacin, difloxacin, enrofloxacin, ciprofloxacin, fleroxacin, dafloxacin, pefloxacin, lomefloxacin and salafloxacin in bean sprouts by colloidal gold immunochromatography.Methods Took representative bean sprout ground 2 g, extracted with 5 mL extraction solution, and then filtered and diluted 2 times. Drew the diluent into the gold standard micropore, incubated at room temperature, and inserted the test strip into the gold standard micropore. The reaction results were interpreted by comparing the color depth of the detection line (T line) and the control line (C line). The experiment also studied the cross-reaction of 6-benzyladenine, 4-chlorophenoxyacetic acid, metronidazole, urea and clopidourea, and the reliability of the method was investigated by real positive samples and multi-level spiked samples.Results The detection limit of the method could reach 5 μg/kg ; no cross reactions occurred with 6-benzyladenine, 4-chlorophenoxyacetic acid, metronidazole, urea and clopidourea under the condition of routine detection level; The results of real positive samples and multi-level spiked samples confirmed that the method was reliable.Conclusion The method is sensitive, accurate and fast, which is suitable for the field rapid detection of quinolones in bean sprouts.

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刘美,姚欢,余晓琴,夏梦婷.胶体金试纸条在豆芽喹诺酮快速检测的应用[J].中国食品卫生杂志,2023,35(12):1722-1726.

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  • 收稿日期:2022-06-20
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  • 在线发布日期: 2024-04-03
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