Objective To analyze the serotype distribution, drug resistance and molecular typing characteristics of salmonella isolates from infectious diarrhea patients in Huzhou City, and to provide scientific basis for the prevention and control, early warning, tracing and clinical treatment of diarrheal diseases caused by salmonella. Methods 126 isolates of Salmonella were collected from 6 sentinel hospitals in Huzhou City during 2021-2022.The isolates were characterized by serovar determination, antimicrobial susceptibility tests, and pulse-eld gel electrophoresis (PFGE) typing. Results The 126 strains of Salmonella could be divided into 18 serotypes, the dominant serotypes were Salmonella typhimurium (47.6%, 60/126) and Salmonella enteritidis (24.6%, 31/126) . The resistance rate of salmonella typhimurium to ampicillin and sulfamethoxazole was the highest (56.7%), and Salmonella enteritidis to ampicillin/sulbactam was the highest of 54.8%, and that of other salmonella to tetracycline was the highest of 51.4%. The overall multidrug resistance rate was 62.7% (79/126), showing 20 multidrug resistance spectra. The PFGE fingerprints of Salmonella typhimurium and Salmonella enteritidis were obtained by XbaⅠ enzyme. Conclusion The predominant serotypes of salmonella infection in Huzhou city are Salmonella typhimurium and Salmonella enteritidis. The multi-drug resistance of salmonella bacteria is serious. PFGE zone types are both diverse and dominant.