Objective: The aim of this study was to explore the drug resistance spectrum and drug resistance characteristics of Salmonella enteritidis in Lanzhou and establish a database of some genotypes of Salmonella to provide scientific value for effective prevention of Salmonella infection and treatment.Methods: Firstly, 94 strains of Salmonella were isolated from foodborne active surveillance in Lanzhou City, 2019–2021 by serotyping and biochemical analysis; Furthermore, the drug resistance of 41 strains of Salmonella enteritidis was detected by drug susceptibility testing and pulsed field gel electrophoresis; Finally, BioNumerics (Version 6.0) software was used to cluster the PFGE profiles of 41 strains of Salmonella enteritidis. Results:Firstly, we found that 94 strains of Salmonella could be classified into 20 serotypes, with Salmonella enteritidis as the dominant serogroup, accounting for 43.62% of the total (41/94). Then, the 41 strains of Salmonella enteritidis were generally resistant to quinolone nalidixic acid bacteria (100.00%), followed by up to 80.49% resistance to penicillin ampicillin,the resistance rate to cefazolin was 39.02%,and the resistance rates to ampicillin/sulbactam and tetracycline were 31.71%. But they were all sensitive to imipenem and cefoxitin; 26 (63.41%) of the strains showed multi–drug resistance, resulting in five multi–drug resistance profiles. Lastly, A total of 24 bands were obtained by Xba I digestion of 41 strains of Salmonella enteritidis, and the similarity between the bands was 26.92%–97.14%. The number of strains contained in each band type was different, and the homology between strains of the same year was highly. Conclusion: Salmonella serotypes in Lanzhou are diverse and PFGE band types are complex, and its dominant serogroup Salmonella enteritidis is highly resistant and multi–drug resistant seriously.