Objective To develop a rapid method for detecting residues of 13 quinolones in aquatic products by liquid chromatography-tandem mass spectrometry. Methods The chromatographic column was Ultimate XB-C₁₈（2.1 mm×150 mm，5 μm）. The optimal analytical conditions and detection parameters were confirmed. The electrospray conditions were as follows: ion source, ESI(+); selection reaction monitoring (SRM) system; ion-spray voltage, 4500 V; sheath gas pressure, 35 units; auxiliary gas pressure, 5 units; ion source temperature, 350℃; collision induced dissociation (CID) pressure, 10 V. And the best conditions and procedures for extraction and purification were established. Results The average recovery was 74.6%-113.0%, and the relative standard deviation was 3.24%-12.11% (n=6), the limit of quantification (LOQ) was 1.00 μg/kg. The linear range was 3.0-200.0 ng/ml. Conclusion This method was suitable for the detection of 13 quinolones residues in aquatic products.